Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H2785_RS15245 Genome accession   NZ_CP059546
Coordinates   3272213..3273013 (-) Length   266 a.a.
NCBI ID   WP_193759251.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 10_4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3267213..3278013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2785_RS15225 (H2785_15230) - 3268278..3268976 (-) 699 WP_000058788.1 YfgM family protein -
  H2785_RS15230 (H2785_15235) hisS 3269005..3270297 (-) 1293 WP_000095252.1 histidine--tRNA ligase -
  H2785_RS15235 (H2785_15240) ispG 3270294..3271409 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H2785_RS15240 (H2785_15245) - 3271431..3272222 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  H2785_RS15245 (H2785_15250) pilF 3272213..3273013 (-) 801 WP_193759251.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H2785_RS15250 (H2785_15255) rlmN 3273029..3274261 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H2785_RS15255 (H2785_15260) ndk 3274384..3274815 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  H2785_RS15260 (H2785_15265) iscX 3274929..3275129 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  H2785_RS15265 (H2785_15270) - 3275229..3275780 (-) 552 WP_000669566.1 phosphatase PAP2 family protein -
  H2785_RS15270 (H2785_15275) - 3275805..3277103 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30110.44 Da        Isoelectric Point: 9.2307

>NTDB_id=470366 H2785_RS15245 WP_193759251.1 3272213..3273013(-) (pilF) [Acinetobacter baumannii strain 10_4]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=470366 H2785_RS15245 WP_193759251.1 3272213..3273013(-) (pilF) [Acinetobacter baumannii strain 10_4]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTGTTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722