Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H2N97_RS01750 Genome accession   NZ_CP059497
Coordinates   332299..332736 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain JSRB 08     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 327299..337736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2N97_RS01725 (H2N97_01725) - 328320..329456 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  H2N97_RS01730 (H2N97_01730) - 329470..329904 (+) 435 WP_061861834.1 RDD family protein -
  H2N97_RS01735 (H2N97_01735) - 330021..330299 (+) 279 WP_263612330.1 YckD family protein -
  H2N97_RS01740 (H2N97_01740) - 330403..331839 (+) 1437 WP_134986369.1 glycoside hydrolase family 1 protein -
  H2N97_RS01745 (H2N97_01745) nin/comJ 331880..332278 (-) 399 WP_014416873.1 competence protein ComJ Regulator
  H2N97_RS01750 (H2N97_01750) nucA/comI 332299..332736 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H2N97_RS01755 (H2N97_01755) hxlB 333067..333624 (-) 558 WP_134986370.1 6-phospho-3-hexuloisomerase -
  H2N97_RS01760 (H2N97_01760) hxlA 333621..334256 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  H2N97_RS01765 (H2N97_01765) - 334488..334850 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=470181 H2N97_RS01750 WP_003156588.1 332299..332736(-) (nucA/comI) [Bacillus velezensis strain JSRB 08]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=470181 H2N97_RS01750 WP_003156588.1 332299..332736(-) (nucA/comI) [Bacillus velezensis strain JSRB 08]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641