Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   H2N74_RS18130 Genome accession   NZ_CP059495
Coordinates   3707661..3708806 (+) Length   381 a.a.
NCBI ID   WP_014419129.1    Uniprot ID   -
Organism   Bacillus velezensis strain JSRB 166     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3702661..3713806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2N74_RS18110 (H2N74_18110) - 3703136..3703852 (-) 717 WP_007407631.1 Crp/Fnr family transcriptional regulator -
  H2N74_RS18115 (H2N74_18115) narK 3703950..3705140 (-) 1191 WP_022553931.1 nitrate transporter NarK -
  H2N74_RS18120 (H2N74_18120) argS 3705274..3706944 (-) 1671 WP_007407633.1 arginine--tRNA ligase -
  H2N74_RS18125 (H2N74_18125) - 3706941..3707369 (-) 429 WP_014419128.1 DUF1934 domain-containing protein -
  H2N74_RS18130 (H2N74_18130) rapF 3707661..3708806 (+) 1146 WP_014419129.1 tetratricopeptide repeat protein Regulator
  H2N74_RS18135 (H2N74_18135) - 3708790..3708909 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  H2N74_RS18140 (H2N74_18140) - 3709107..3709310 (+) 204 WP_029972878.1 helix-turn-helix transcriptional regulator -
  H2N74_RS18145 (H2N74_18145) - 3709324..3709527 (+) 204 WP_007407641.1 hypothetical protein -
  H2N74_RS18150 (H2N74_18150) - 3709562..3709747 (+) 186 WP_014419130.1 DUF4177 domain-containing protein -
  H2N74_RS18155 (H2N74_18155) - 3709934..3710215 (+) 282 WP_014419131.1 hypothetical protein -
  H2N74_RS18160 (H2N74_18160) - 3710259..3710771 (+) 513 WP_014419132.1 stage II sporulation protein M -
  H2N74_RS18165 (H2N74_18165) - 3711362..3711580 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  H2N74_RS18170 (H2N74_18170) speB 3711733..3712605 (-) 873 WP_053285340.1 agmatinase -
  H2N74_RS18175 (H2N74_18175) speE 3712664..3713494 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45581.65 Da        Isoelectric Point: 4.5797

>NTDB_id=470169 H2N74_RS18130 WP_014419129.1 3707661..3708806(+) (rapF) [Bacillus velezensis strain JSRB 166]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSELLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=470169 H2N74_RS18130 WP_014419129.1 3707661..3708806(+) (rapF) [Bacillus velezensis strain JSRB 166]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGAGCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.215

100

0.782
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57