Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H2N74_RS01760 Genome accession   NZ_CP059495
Coordinates   332525..332962 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain JSRB 166     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 327525..337962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2N74_RS01735 (H2N74_01735) - 328545..329681 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  H2N74_RS01740 (H2N74_01740) - 329696..330130 (+) 435 WP_014416871.1 RDD family protein -
  H2N74_RS01745 (H2N74_01745) - 330247..330525 (+) 279 WP_374978596.1 YckD family protein -
  H2N74_RS01750 (H2N74_01750) - 330629..332065 (+) 1437 WP_174532722.1 glycoside hydrolase family 1 protein -
  H2N74_RS01755 (H2N74_01755) nin/comJ 332106..332504 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  H2N74_RS01760 (H2N74_01760) nucA/comI 332525..332962 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H2N74_RS01765 (H2N74_01765) hxlB 333293..333850 (-) 558 WP_061861836.1 6-phospho-3-hexuloisomerase -
  H2N74_RS01770 (H2N74_01770) hxlA 333847..334482 (-) 636 WP_182072283.1 3-hexulose-6-phosphate synthase -
  H2N74_RS01775 (H2N74_01775) - 334714..335076 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=470105 H2N74_RS01760 WP_003156588.1 332525..332962(-) (nucA/comI) [Bacillus velezensis strain JSRB 166]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=470105 H2N74_RS01760 WP_003156588.1 332525..332962(-) (nucA/comI) [Bacillus velezensis strain JSRB 166]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641