Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JSQ43_RS02325 Genome accession   NZ_CP069709
Coordinates   416575..417252 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411575..422252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSQ43_RS02310 (JSQ43_02310) chiA 412942..415635 (+) 2694 WP_072663100.1 bifunctional chitinase/lysozyme -
  JSQ43_RS02315 (JSQ43_02315) bfd 415804..415998 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JSQ43_RS02320 (JSQ43_02320) bfr 416070..416546 (+) 477 WP_000675504.1 bacterioferritin -
  JSQ43_RS02325 (JSQ43_02325) pilD 416575..417252 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JSQ43_RS02330 (JSQ43_02330) gspM 417252..417713 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JSQ43_RS02335 (JSQ43_02335) gspL 417710..418873 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JSQ43_RS02340 (JSQ43_02340) gspK 418888..419871 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JSQ43_RS02345 (JSQ43_02345) gspJ 419864..420451 (-) 588 WP_112958464.1 type II secretion system minor pseudopilin GspJ -
  JSQ43_RS02350 (JSQ43_02350) gspI 420444..420821 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JSQ43_RS02355 (JSQ43_02355) gspH 420818..421327 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  JSQ43_RS02360 (JSQ43_02360) gspG 421335..421772 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=469908 JSQ43_RS02325 WP_000178161.1 416575..417252(-) (pilD) [Escherichia coli strain EA7]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=469908 JSQ43_RS02325 WP_000178161.1 416575..417252(-) (pilD) [Escherichia coli strain EA7]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378