Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H1W83_RS20610 Genome accession   NZ_CP059457
Coordinates   3919440..3919814 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain 2020WEIHUA_L     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3914440..3924814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W83_RS20590 (H1W83_20435) - 3914935..3915606 (-) 672 WP_034649601.1 response regulator transcription factor -
  H1W83_RS20595 (H1W83_20440) - 3915930..3916748 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  H1W83_RS20600 (H1W83_20445) - 3916784..3918733 (-) 1950 WP_237892811.1 FUSC family protein -
  H1W83_RS20605 (H1W83_20450) - 3918890..3919150 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  H1W83_RS20610 (H1W83_20455) nucA/comI 3919440..3919814 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H1W83_RS20615 (H1W83_20460) comJ 3919850..3920257 (+) 408 WP_016765176.1 competence protein ComJ -
  H1W83_RS20620 (H1W83_20465) helD 3920286..3922604 (-) 2319 WP_237892814.1 RNA polymerase recycling motor HelD -
  H1W83_RS31000 - 3922849..3922974 (-) 126 WP_016765178.1 hypothetical protein -
  H1W83_RS20625 (H1W83_20470) - 3923113..3923628 (-) 516 WP_034655000.1 enoyl-CoA hydratase/isomerase family protein -
  H1W83_RS20630 (H1W83_20475) - 3923871..3924239 (+) 369 WP_016765180.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=469722 H1W83_RS20610 WP_223269005.1 3919440..3919814(+) (nucA/comI) [Priestia megaterium strain 2020WEIHUA_L]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=469722 H1W83_RS20610 WP_223269005.1 3919440..3919814(+) (nucA/comI) [Priestia megaterium strain 2020WEIHUA_L]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGAGAAGAATCCCTGAAAGGATATCCGACAAAAACAGGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGTGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645