Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H1W83_RS17570 Genome accession   NZ_CP059457
Coordinates   3395701..3396123 (+) Length   140 a.a.
NCBI ID   WP_013083984.1    Uniprot ID   A0A1Q8UP88
Organism   Priestia megaterium strain 2020WEIHUA_L     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3390701..3401123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W83_RS17545 (H1W83_17410) - 3390906..3391727 (+) 822 WP_047931982.1 sensor histidine kinase -
  H1W83_RS17550 (H1W83_17415) - 3391728..3392369 (+) 642 WP_034650500.1 response regulator -
  H1W83_RS17555 (H1W83_17420) - 3392546..3393532 (+) 987 WP_237892565.1 glycerophosphodiester phosphodiesterase family protein -
  H1W83_RS17560 (H1W83_17425) - 3393684..3395066 (+) 1383 WP_237892566.1 PepSY-associated TM helix domain-containing protein -
  H1W83_RS17565 (H1W83_17430) - 3395096..3395560 (+) 465 WP_237892567.1 FixH family protein -
  H1W83_RS17570 (H1W83_17435) nucA/comI 3395701..3396123 (+) 423 WP_013083984.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H1W83_RS17575 (H1W83_17440) - 3396278..3396631 (+) 354 WP_234943443.1 DUF1516 family protein -
  H1W83_RS17580 (H1W83_17445) - 3396672..3397265 (-) 594 WP_028409296.1 hypothetical protein -
  H1W83_RS17585 (H1W83_17450) - 3397420..3398127 (-) 708 WP_233334985.1 TetR/AcrR family transcriptional regulator -
  H1W83_RS17590 (H1W83_17455) - 3398099..3399379 (-) 1281 WP_237892568.1 glycosyltransferase -
  H1W83_RS17595 (H1W83_17460) - 3399674..3400219 (+) 546 WP_237892569.1 GNAT family N-acetyltransferase -
  H1W83_RS17600 (H1W83_17465) - 3400517..3401002 (-) 486 WP_171776956.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15356.20 Da        Isoelectric Point: 4.4990

>NTDB_id=469718 H1W83_RS17570 WP_013083984.1 3395701..3396123(+) (nucA/comI) [Priestia megaterium strain 2020WEIHUA_L]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGNGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=469718 H1W83_RS17570 WP_013083984.1 3395701..3396123(+) (nucA/comI) [Priestia megaterium strain 2020WEIHUA_L]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGTGCTGAAGAAAACCGCCGTGAGTCTTTAAAG
GGCATTCCTACTAAAAGCGGCTACGATCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAACGGTGCAGACAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGATCTTGGATCTCACATCAAGTAGACGAGTTTCCAGACGGCACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q8UP88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507