Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M001_RS01640 Genome accession   NZ_CP059396
Coordinates   346571..348010 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii Hm21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341571..353010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M001_RS01610 (M001_001610) mlaF 342148..342948 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  M001_RS01615 (M001_001615) - 343156..344151 (+) 996 WP_021231835.1 KpsF/GutQ family sugar-phosphate isomerase -
  M001_RS01620 (M001_001620) kdsC 344151..344705 (+) 555 WP_021231836.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  M001_RS01625 (M001_001625) lptC 344702..345262 (+) 561 WP_021231837.1 LPS export ABC transporter periplasmic protein LptC -
  M001_RS01630 (M001_001630) lptA 345249..345776 (+) 528 WP_031227941.1 lipopolysaccharide transport periplasmic protein LptA -
  M001_RS01635 (M001_001635) lptB 345780..346505 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  M001_RS01640 (M001_001640) rpoN/rpoN1 346571..348010 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  M001_RS01645 (M001_001645) hpf 348031..348318 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  M001_RS01650 (M001_001650) ptsN 348321..348767 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  M001_RS01655 (M001_001655) rapZ 348813..349679 (+) 867 WP_021231839.1 RNase adapter RapZ -
  M001_RS01660 (M001_001660) npr 349700..349972 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  M001_RS01665 (M001_001665) - 349990..351894 (-) 1905 WP_021231840.1 BglG family transcription antiterminator -
  M001_RS01670 (M001_001670) dagF 352001..352741 (-) 741 WP_021231841.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=469321 M001_RS01640 WP_005336331.1 346571..348010(+) (rpoN/rpoN1) [Aeromonas veronii Hm21]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=469321 M001_RS01640 WP_005336331.1 346571..348010(+) (rpoN/rpoN1) [Aeromonas veronii Hm21]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTACCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACTGCCCAAAGTGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACTACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCTTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCAGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGAGTGCAGCACTT
TGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCTCAGACCCCCT
GGCTCAACGAGGCCAAAGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGTAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGTTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491