Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HU024_RS04650 Genome accession   NZ_CP059344
Coordinates   890725..891339 (+) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain K01     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 885725..896339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU024_RS04615 (HU024_04615) aroE 886411..887253 (+) 843 WP_032873946.1 shikimate dehydrogenase -
  HU024_RS04620 (HU024_04620) yhbY 887247..887537 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  HU024_RS04625 (HU024_04625) - 887548..888117 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  HU024_RS04630 (HU024_04630) yqeK 888107..888682 (+) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HU024_RS04635 (HU024_04635) rsfS 888686..889042 (+) 357 WP_007408260.1 ribosome silencing factor -
  HU024_RS04640 (HU024_04640) - 889039..889776 (+) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  HU024_RS04645 (HU024_04645) comER 889845..890666 (-) 822 WP_012118027.1 late competence protein ComER -
  HU024_RS04650 (HU024_04650) comEA 890725..891339 (+) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  HU024_RS04655 (HU024_04655) - 891406..891975 (+) 570 WP_003152868.1 ComE operon protein 2 -
  HU024_RS04660 (HU024_04660) comEC 891976..894327 (+) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HU024_RS04665 (HU024_04665) - 894346..894480 (-) 135 WP_003152870.1 YqzM family protein -
  HU024_RS04670 (HU024_04670) - 894521..894672 (+) 152 Protein_914 hypothetical protein -
  HU024_RS04675 (HU024_04675) holA 894712..895753 (+) 1042 Protein_915 DNA polymerase III subunit delta -
  HU024_RS04680 (HU024_04680) rpsT 895770..896036 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=468851 HU024_RS04650 WP_032873955.1 890725..891339(+) (comEA) [Bacillus velezensis strain K01]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=468851 HU024_RS04650 WP_032873955.1 890725..891339(+) (comEA) [Bacillus velezensis strain K01]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363