Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JSQ89_RS10545 Genome accession   NZ_CP069627
Coordinates   2205481..2206158 (+) Length   225 a.a.
NCBI ID   WP_000178174.1    Uniprot ID   A0A0H2VBG7
Organism   Escherichia coli strain ESM1.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2200481..2211158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSQ89_RS10510 (JSQ89_10405) gspG 2200961..2201398 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  JSQ89_RS10515 (JSQ89_10410) gspH 2201406..2201915 (+) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  JSQ89_RS10520 (JSQ89_10415) gspI 2201912..2202289 (+) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  JSQ89_RS10525 (JSQ89_10420) gspJ 2202282..2202869 (+) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  JSQ89_RS10530 (JSQ89_10425) gspK 2202862..2203845 (+) 984 Protein_2065 type II secretion system minor pseudopilin GspK -
  JSQ89_RS10535 (JSQ89_10430) gspL 2203860..2205023 (+) 1164 WP_001304915.1 type II secretion system protein GspL -
  JSQ89_RS10540 (JSQ89_10435) gspM 2205020..2205481 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  JSQ89_RS10545 (JSQ89_10440) pilD 2205481..2206158 (+) 678 WP_000178174.1 prepilin peptidase GspO Machinery gene
  JSQ89_RS10550 (JSQ89_10445) bfr 2206187..2206663 (-) 477 WP_000675504.1 bacterioferritin -
  JSQ89_RS10555 (JSQ89_10450) bfd 2206735..2206929 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JSQ89_RS10560 (JSQ89_10455) chiA 2207098..2209800 (-) 2703 WP_000773147.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24866.76 Da        Isoelectric Point: 7.6364

>NTDB_id=468830 JSQ89_RS10545 WP_000178174.1 2205481..2206158(+) (pilD) [Escherichia coli strain ESM1.4]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=468830 JSQ89_RS10545 WP_000178174.1 2205481..2206158(+) (pilD) [Escherichia coli strain ESM1.4]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387