Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J80_RS07785 Genome accession   NZ_CP069571
Coordinates   1538389..1539066 (-) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1533389..1544066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J80_RS07770 (I6J80_07770) chiA 1534756..1537449 (+) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -
  I6J80_RS07775 (I6J80_07775) bfd 1537618..1537812 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J80_RS07780 (I6J80_07780) bfr 1537884..1538360 (+) 477 WP_000675504.1 bacterioferritin -
  I6J80_RS07785 (I6J80_07785) pilD 1538389..1539066 (-) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  I6J80_RS07790 (I6J80_07790) gspM 1539066..1539527 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  I6J80_RS07795 (I6J80_07795) gspL 1539524..1540687 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  I6J80_RS07800 (I6J80_07800) gspK 1540702..1541685 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  I6J80_RS07805 (I6J80_07805) gspJ 1541678..1542265 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  I6J80_RS07810 (I6J80_07810) gspI 1542258..1542635 (-) 378 WP_167814358.1 type II secretion system minor pseudopilin GspI -
  I6J80_RS07815 (I6J80_07815) gspH 1542632..1543141 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  I6J80_RS07820 (I6J80_07820) gspG 1543149..1543586 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=468401 I6J80_RS07785 WP_000178163.1 1538389..1539066(-) (pilD) [Escherichia coli strain FDAARGOS_1250]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=468401 I6J80_RS07785 WP_000178163.1 1538389..1539066(-) (pilD) [Escherichia coli strain FDAARGOS_1250]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378