Detailed information NTG0468401

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H0529_RS11190
• Genome accession: NZ_CP059300
• Coordinates: 2312965..2314083 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AC1633
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2307965..2319083

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0529_RS11180 (H0529_11180)	-	2311081..2311773 (-)	693	WP_031965550.1	YggS family pyridoxal phosphate-dependent enzyme	-
H0529_RS11185 (H0529_11185)	pilT	2311900..2312937 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H0529_RS11190 (H0529_11190)	pilU	2312965..2314083 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H0529_RS11195 (H0529_11195)	fur	2314152..2314589 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H0529_RS11200 (H0529_11200)	-	2314701..2315099 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H0529_RS11205 (H0529_11205)	-	2315154..2315477 (-)	324	WP_000442585.1	RnfH family protein	-
H0529_RS11210 (H0529_11210)	-	2315477..2316544 (-)	1068	WP_002084046.1	hypothetical protein	-
H0529_RS11215 (H0529_11215)	-	2316697..2317152 (-)	456	WP_000782976.1	bacteriohemerythrin	-
H0529_RS11220 (H0529_11220)	-	2317405..2318253 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=468401 H0529_RS11190 WP_000347036.1 2312965..2314083(+) (pilU) [Acinetobacter baumannii strain AC1633]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=468401 H0529_RS11190 WP_000347036.1 2312965..2314083(+) (pilU) [Acinetobacter baumannii strain AC1633]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCAGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTTGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392