Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J89_RS03855 Genome accession   NZ_CP069559
Coordinates   829316..829993 (-) Length   225 a.a.
NCBI ID   WP_000178172.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1259     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824316..834993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J89_RS03840 (I6J89_03840) chiA 825677..828376 (+) 2700 WP_000773163.1 bifunctional chitinase/lysozyme -
  I6J89_RS03845 (I6J89_03845) bfd 828545..828739 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J89_RS03850 (I6J89_03850) bfr 828811..829287 (+) 477 WP_000675504.1 bacterioferritin -
  I6J89_RS03855 (I6J89_03855) pilD 829316..829993 (-) 678 WP_000178172.1 prepilin peptidase GspO Machinery gene
  I6J89_RS03860 (I6J89_03860) gspM 829993..830454 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J89_RS03865 (I6J89_03865) gspL 830451..831614 (-) 1164 WP_001304915.1 type II secretion system protein GspL -
  I6J89_RS03870 (I6J89_03870) gspK 831629..832611 (-) 983 Protein_757 type II secretion system minor pseudopilin GspK -
  I6J89_RS03875 (I6J89_03875) gspJ 832604..833191 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  I6J89_RS03880 (I6J89_03880) gspI 833184..833561 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  I6J89_RS03885 (I6J89_03885) gspH 833558..834067 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  I6J89_RS03890 (I6J89_03890) gspG 834075..834512 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.70 Da        Isoelectric Point: 7.6364

>NTDB_id=468219 I6J89_RS03855 WP_000178172.1 829316..829993(-) (pilD) [Escherichia coli strain FDAARGOS_1259]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTAPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=468219 I6J89_RS03855 WP_000178172.1 829316..829993(-) (pilD) [Escherichia coli strain FDAARGOS_1259]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGCGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382