Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS074_RS09555 Genome accession   NZ_CP059245
Coordinates   1886336..1886815 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0740     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1881336..1891815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS074_RS09530 (HS074_09455) - 1881919..1882683 (-) 765 WP_409038309.1 TSUP family transporter -
  HS074_RS09535 (HS074_09460) proB 1882683..1883768 (-) 1086 WP_409037686.1 glutamate 5-kinase -
  HS074_RS09540 (HS074_09465) folA 1883880..1884374 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS074_RS09545 (HS074_09470) - 1884493..1884723 (+) 231 WP_006248398.1 YgjV family protein -
  HS074_RS09550 (HS074_09475) radA 1884778..1886157 (-) 1380 WP_240019414.1 DNA repair protein RadA -
  HS074_RS09555 (HS074_09480) pilA 1886336..1886815 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS074_RS09560 (HS074_09485) - 1886836..1887279 (+) 444 Protein_1837 pilus assembly protein PilB -
  HS074_RS09565 (HS074_09490) pilC 1887285..1888484 (+) 1200 WP_409038310.1 type II secretion system F family protein Machinery gene
  HS074_RS09570 - 1888487..1888690 (+) 204 WP_240019416.1 hypothetical protein -
  HS074_RS09575 - 1888762..1888968 (+) 207 Protein_1840 peptidase A24 -
  HS074_RS09580 (HS074_09500) coaE 1889182..1889817 (+) 636 WP_409038838.1 dephospho-CoA kinase -
  HS074_RS09585 (HS074_09505) yacG 1889799..1889984 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS074_RS09590 (HS074_09510) holA 1889985..1891010 (-) 1026 WP_409038839.1 DNA polymerase III subunit delta -
  HS074_RS09595 (HS074_09515) lptE 1891053..1891541 (-) 489 WP_409038311.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=468151 HS074_RS09555 WP_006248401.1 1886336..1886815(+) (pilA) [Mannheimia haemolytica strain 0740]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=468151 HS074_RS09555 WP_006248401.1 1886336..1886815(+) (pilA) [Mannheimia haemolytica strain 0740]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491