Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J97_RS01725 Genome accession   NZ_CP069522
Coordinates   358265..358942 (-) Length   225 a.a.
NCBI ID   WP_000178166.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1267     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 353265..363942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J97_RS01710 (I6J97_01710) chiA 354623..357325 (+) 2703 WP_000773168.1 bifunctional chitinase/lysozyme -
  I6J97_RS01715 (I6J97_01715) bfd 357494..357688 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J97_RS01720 (I6J97_01720) bfr 357760..358236 (+) 477 WP_000675504.1 bacterioferritin -
  I6J97_RS01725 (I6J97_01725) pilD 358265..358942 (-) 678 WP_000178166.1 prepilin peptidase GspO Machinery gene
  I6J97_RS01730 (I6J97_01730) gspM 358942..359403 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J97_RS01735 (I6J97_01735) gspL 359400..360563 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  I6J97_RS01740 (I6J97_01740) gspK 360578..361561 (-) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -
  I6J97_RS01745 (I6J97_01745) gspJ 361554..362141 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  I6J97_RS01750 (I6J97_01750) gspI 362134..362511 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  I6J97_RS01755 (I6J97_01755) gspH 362508..363017 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  I6J97_RS01760 (I6J97_01760) gspG 363025..363462 (-) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24814.64 Da        Isoelectric Point: 7.6364

>NTDB_id=467748 I6J97_RS01725 WP_000178166.1 358265..358942(-) (pilD) [Escherichia coli strain FDAARGOS_1267]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLAHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=467748 I6J97_RS01725 WP_000178166.1 358265..358942(-) (pilD) [Escherichia coli strain FDAARGOS_1267]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGCTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382