Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J88_RS11885 Genome accession   NZ_CP069500
Coordinates   2371861..2372538 (+) Length   225 a.a.
NCBI ID   WP_000178172.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1258     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2366861..2377538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J88_RS11850 (I6J88_11855) gspG 2367341..2367778 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6J88_RS11855 (I6J88_11860) gspH 2367786..2368295 (+) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  I6J88_RS11860 (I6J88_11865) gspI 2368292..2368669 (+) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  I6J88_RS11865 (I6J88_11870) gspJ 2368662..2369249 (+) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  I6J88_RS11870 (I6J88_11875) gspK 2369242..2370225 (+) 984 WP_001058529.1 type II secretion system minor pseudopilin GspK -
  I6J88_RS11875 (I6J88_11880) gspL 2370240..2371403 (+) 1164 WP_096970235.1 type II secretion system protein GspL -
  I6J88_RS11880 (I6J88_11885) gspM 2371400..2371861 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J88_RS11885 (I6J88_11890) pilD 2371861..2372538 (+) 678 WP_000178172.1 prepilin peptidase GspO Machinery gene
  I6J88_RS11890 (I6J88_11895) bfr 2372567..2373043 (-) 477 WP_000675504.1 bacterioferritin -
  I6J88_RS11895 (I6J88_11900) bfd 2373115..2373309 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J88_RS11900 (I6J88_11905) chiA 2373478..2376177 (-) 2700 WP_000773163.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.70 Da        Isoelectric Point: 7.6364

>NTDB_id=467519 I6J88_RS11885 WP_000178172.1 2371861..2372538(+) (pilD) [Escherichia coli strain FDAARGOS_1258]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTAPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=467519 I6J88_RS11885 WP_000178172.1 2371861..2372538(+) (pilD) [Escherichia coli strain FDAARGOS_1258]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGCGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382