Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H0242_RS20495 Genome accession   NZ_CP059148
Coordinates   4039955..4040389 (-) Length   144 a.a.
NCBI ID   WP_000811508.1    Uniprot ID   A0A9X8M3D6
Organism   Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4034955..4045389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0242_RS20480 (H0242_20235) - 4036132..4036689 (+) 558 WP_086407639.1 PadR family transcriptional regulator -
  H0242_RS20485 (H0242_20240) metE 4037202..4039490 (+) 2289 WP_001007611.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  H0242_RS20490 (H0242_20245) - 4039529..4039918 (-) 390 WP_000424036.1 competence protein ComJ -
  H0242_RS20495 (H0242_20250) nucA/comI 4039955..4040389 (-) 435 WP_000811508.1 DNA-entry nuclease Machinery gene
  H0242_RS20500 (H0242_20255) - 4040618..4041367 (+) 750 WP_000388465.1 DUF3967 domain-containing protein -
  H0242_RS20505 (H0242_20260) kinB 4041406..4042680 (-) 1275 WP_000420361.1 sporulation sensor histidine kinase KinB -
  H0242_RS20510 (H0242_20265) - 4042991..4043191 (+) 201 WP_000929277.1 hypothetical protein -
  H0242_RS20515 (H0242_20270) - 4043314..4044477 (+) 1164 WP_000434600.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16040.40 Da        Isoelectric Point: 8.4331

>NTDB_id=467376 H0242_RS20495 WP_000811508.1 4039955..4040389(-) (nucA/comI) [Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=467376 H0242_RS20495 WP_000811508.1 4039955..4040389(-) (nucA/comI) [Bacillus thuringiensis serovar sumiyoshiensis strain 4AO1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTGTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCTTCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549