Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   I6J87_RS15975 Genome accession   NZ_CP069480
Coordinates   3308352..3309011 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain FDAARGOS_1257     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3303352..3314011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J87_RS15950 (I6J87_15950) ygiN 3304253..3304567 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  I6J87_RS15955 (I6J87_15955) mdaB 3304598..3305179 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  I6J87_RS25695 - 3305429..3305908 (+) 480 WP_000065329.1 Hcp family type VI secretion system effector -
  I6J87_RS25700 - 3305911..3306621 (+) 711 WP_000834021.1 hypothetical protein -
  I6J87_RS15965 (I6J87_15965) ygiZ 3306628..3306960 (+) 333 WP_000914695.1 DUF2645 family protein -
  I6J87_RS15970 (I6J87_15970) qseC 3307006..3308355 (-) 1350 WP_000673391.1 quorum sensing histidine kinase QseC -
  I6J87_RS15975 (I6J87_15975) ciaR 3308352..3309011 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  I6J87_RS15980 (I6J87_15980) ygiW 3309163..3309555 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  I6J87_RS15985 (I6J87_15985) ygiV 3309608..3310090 (+) 483 WP_000183491.1 GyrI-like domain-containing protein -
  I6J87_RS15990 (I6J87_15990) ygiS 3310199..3311806 (+) 1608 WP_001298384.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=467366 I6J87_RS15975 WP_001221493.1 3308352..3309011(-) (ciaR) [Escherichia coli strain FDAARGOS_1257]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=467366 I6J87_RS15975 WP_001221493.1 3308352..3309011(-) (ciaR) [Escherichia coli strain FDAARGOS_1257]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TAGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGAAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365