Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H0S56_RS11965 Genome accession   NZ_CP059081
Coordinates   2502760..2503560 (-) Length   266 a.a.
NCBI ID   WP_044109965.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain 12CE1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497760..2508560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S56_RS11945 (H0S56_11940) - 2498805..2499503 (-) 699 WP_044109972.1 tetratricopeptide repeat protein -
  H0S56_RS11950 (H0S56_11945) hisS 2499534..2500829 (-) 1296 WP_044109970.1 histidine--tRNA ligase -
  H0S56_RS11955 (H0S56_11950) ispG 2500833..2501954 (-) 1122 WP_004278355.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H0S56_RS11960 (H0S56_11955) - 2501978..2502769 (-) 792 WP_004647463.1 DUF4115 domain-containing protein -
  H0S56_RS11965 (H0S56_11960) pilF 2502760..2503560 (-) 801 WP_044109965.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H0S56_RS11970 (H0S56_11965) rlmN 2503575..2504810 (-) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H0S56_RS11975 (H0S56_11970) ndk 2504933..2505364 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  H0S56_RS11980 (H0S56_11975) iscX 2505475..2505675 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  H0S56_RS11985 (H0S56_11980) - 2505763..2506314 (-) 552 WP_004278361.1 phosphatase PAP2 family protein -
  H0S56_RS11990 (H0S56_11985) - 2506335..2507615 (-) 1281 WP_004647459.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30149.26 Da        Isoelectric Point: 9.2878

>NTDB_id=467135 H0S56_RS11965 WP_044109965.1 2502760..2503560(-) (pilF) [Acinetobacter lwoffii strain 12CE1]
MRNLTSKFTLISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=467135 H0S56_RS11965 WP_044109965.1 2502760..2503560(-) (pilF) [Acinetobacter lwoffii strain 12CE1]
TTGAGAAATCTAACATCAAAGTTTACTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCACAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGTGCATTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

97.744

0.767
  pilF Acinetobacter baylyi ADP1

69.925

100

0.699