Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H0S57_RS14235 Genome accession   NZ_CP059080
Coordinates   2954541..2955341 (-) Length   266 a.a.
NCBI ID   WP_004697674.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain E10B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2949541..2960341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0S57_RS14215 (H0S57_14220) - 2950622..2951320 (-) 699 WP_151836405.1 tetratricopeptide repeat protein -
  H0S57_RS14220 (H0S57_14225) hisS 2951348..2952643 (-) 1296 WP_062031308.1 histidine--tRNA ligase -
  H0S57_RS14225 (H0S57_14230) ispG 2952640..2953755 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H0S57_RS14230 (H0S57_14235) - 2953774..2954550 (-) 777 WP_151836404.1 helix-turn-helix domain-containing protein -
  H0S57_RS14235 (H0S57_14240) pilF 2954541..2955341 (-) 801 WP_004697674.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H0S57_RS14240 (H0S57_14245) rlmN 2955358..2956593 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H0S57_RS14245 (H0S57_14250) ndk 2956716..2957147 (-) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  H0S57_RS14250 (H0S57_14255) iscX 2957258..2957458 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  H0S57_RS14255 (H0S57_14260) - 2957549..2958100 (-) 552 WP_004697666.1 phosphatase PAP2 family protein -
  H0S57_RS14260 (H0S57_14265) - 2958293..2959573 (-) 1281 WP_004697665.1 glycosyltransferase family 1 protein -
  H0S57_RS14265 (H0S57_14270) - 2959714..2960130 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=467095 H0S57_RS14235 WP_004697674.1 2954541..2955341(-) (pilF) [Acinetobacter johnsonii strain E10B]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=467095 H0S57_RS14235 WP_004697674.1 2954541..2955341(-) (pilF) [Acinetobacter johnsonii strain E10B]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGGGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCTGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCCGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTGGGCGAGAAAAACTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGATAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647