Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J86_RS22490 Genome accession   NZ_CP069451
Coordinates   4721190..4721867 (+) Length   225 a.a.
NCBI ID   WP_000178177.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1256     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4716190..4726867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J86_RS22455 (I6J86_22455) gspG 4716652..4717089 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6J86_RS22460 (I6J86_22460) gspH 4717097..4717606 (+) 510 WP_001076037.1 type II secretion system minor pseudopilin GspH -
  I6J86_RS22465 (I6J86_22465) gspI 4717603..4717998 (+) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  I6J86_RS22470 (I6J86_22470) gspJ 4717991..4718578 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I6J86_RS22475 (I6J86_22475) gspK 4718571..4719554 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  I6J86_RS22480 (I6J86_22480) gspL 4719569..4720732 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  I6J86_RS22485 (I6J86_22485) gspM 4720729..4721190 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J86_RS22490 (I6J86_22490) pilD 4721190..4721867 (+) 678 WP_000178177.1 prepilin peptidase GspO Machinery gene
  I6J86_RS22495 (I6J86_22495) bfr 4721896..4722372 (-) 477 WP_000675504.1 bacterioferritin -
  I6J86_RS22500 (I6J86_22500) bfd 4722444..4722638 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J86_RS22505 (I6J86_22505) chiA 4722807..4725509 (-) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24900.77 Da        Isoelectric Point: 7.6364

>NTDB_id=467046 I6J86_RS22490 WP_000178177.1 4721190..4721867(+) (pilD) [Escherichia coli strain FDAARGOS_1256]
MTMLLPLFILVGFIASYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=467046 I6J86_RS22490 WP_000178177.1 4721190..4721867(+) (pilD) [Escherichia coli strain FDAARGOS_1256]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAAGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382