Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   I6J86_RS04735 Genome accession   NZ_CP069451
Coordinates   1001238..1002620 (+) Length   460 a.a.
NCBI ID   WP_042193805.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1256     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 996238..1007620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J86_RS04710 (I6J86_04710) deoD 997252..997971 (+) 720 WP_000224879.1 purine-nucleoside phosphorylase -
  I6J86_RS04715 (I6J86_04715) - 998153..998395 (-) 243 WP_000566144.1 helix-turn-helix transcriptional regulator -
  I6J86_RS04720 (I6J86_04720) lplA 998427..999443 (-) 1017 WP_000105872.1 lipoate--protein ligase LplA -
  I6J86_RS04725 (I6J86_04725) ytjB 999471..1000115 (-) 645 WP_000124608.1 YtjB family periplasmic protein -
  I6J86_RS04730 (I6J86_04730) serB 1000221..1001189 (+) 969 WP_001132964.1 phosphoserine phosphatase -
  I6J86_RS04735 (I6J86_04735) radA/sms 1001238..1002620 (+) 1383 WP_042193805.1 DNA repair protein RadA Machinery gene
  I6J86_RS04740 (I6J86_04740) nadR 1002641..1003873 (+) 1233 WP_000093832.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  I6J86_RS04745 (I6J86_04745) - 1003965..1004297 (+) 333 WP_001298496.1 type II toxin-antitoxin system RelE/ParE family toxin -
  I6J86_RS04750 (I6J86_04750) nadS 1004299..1004583 (+) 285 WP_000007436.1 NadS family protein -
  I6J86_RS04755 (I6J86_04755) ettA 1004639..1006306 (-) 1668 WP_000046754.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49486.07 Da        Isoelectric Point: 7.1936

>NTDB_id=466991 I6J86_RS04735 WP_042193805.1 1001238..1002620(+) (radA/sms) [Escherichia coli strain FDAARGOS_1256]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGSVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSVDLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=466991 I6J86_RS04735 WP_042193805.1 1001238..1002620(+) (radA/sms) [Escherichia coli strain FDAARGOS_1256]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGCGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTTAATATGCTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAATCATCACCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTCGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGTGATGCCGACTCACGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCTCGGTGAATGAGCT
GGGTGTCTTCGCAATGACCGAGCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTAGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATTCAGGCGCTCGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCAGTCGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTTCACCG
TCACGGTGGTCTACAAATGGCCGATCAGGATGTGTTTGTTAACGTGGTCGGTGGCGTGAAGGTGACGGAAACCAGTGTCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
AATTGTTCCGGCAGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

47.046

99.348

0.467
  radA Streptococcus mitis NCTC 12261

43.64

99.13

0.433
  radA Streptococcus pneumoniae Rx1

43.64

99.13

0.433
  radA Streptococcus pneumoniae D39

43.64

99.13

0.433
  radA Streptococcus pneumoniae R6

43.64

99.13

0.433
  radA Streptococcus pneumoniae TIGR4

43.64

99.13

0.433
  radA Streptococcus mitis SK321

43.64

99.13

0.433