Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J90_RS11955 Genome accession   NZ_CP069438
Coordinates   2421946..2422623 (+) Length   225 a.a.
NCBI ID   WP_000178172.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1260     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2416946..2427623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J90_RS11920 (I6J90_11920) gspG 2417427..2417864 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6J90_RS11925 (I6J90_11925) gspH 2417872..2418381 (+) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  I6J90_RS11930 (I6J90_11930) gspI 2418378..2418755 (+) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  I6J90_RS11935 (I6J90_11935) gspJ 2418748..2419335 (+) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  I6J90_RS11940 (I6J90_11940) gspK 2419328..2420310 (+) 983 Protein_2366 type II secretion system minor pseudopilin GspK -
  I6J90_RS11945 (I6J90_11945) gspL 2420325..2421488 (+) 1164 WP_001304915.1 type II secretion system protein GspL -
  I6J90_RS11950 (I6J90_11950) gspM 2421485..2421946 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J90_RS11955 (I6J90_11955) pilD 2421946..2422623 (+) 678 WP_000178172.1 prepilin peptidase GspO Machinery gene
  I6J90_RS11960 (I6J90_11960) bfr 2422652..2423128 (-) 477 WP_000675504.1 bacterioferritin -
  I6J90_RS11965 (I6J90_11965) bfd 2423200..2423394 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J90_RS11970 (I6J90_11970) chiA 2423563..2426262 (-) 2700 WP_000773163.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.70 Da        Isoelectric Point: 7.6364

>NTDB_id=466651 I6J90_RS11955 WP_000178172.1 2421946..2422623(+) (pilD) [Escherichia coli strain FDAARGOS_1260]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTAPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=466651 I6J90_RS11955 WP_000178172.1 2421946..2422623(+) (pilD) [Escherichia coli strain FDAARGOS_1260]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGCGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382