Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HYO64_RS16070 Genome accession   NZ_CP058729
Coordinates   3348576..3349376 (-) Length   266 a.a.
NCBI ID   WP_213067660.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain M164-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3343576..3354376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYO64_RS16050 - 3344641..3345339 (-) 699 WP_114155372.1 YfgM family protein -
  HYO64_RS16055 hisS 3345368..3346660 (-) 1293 WP_114155370.1 histidine--tRNA ligase -
  HYO64_RS16060 ispG 3346657..3347772 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  HYO64_RS16065 - 3347794..3348585 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  HYO64_RS16070 pilF 3348576..3349376 (-) 801 WP_213067660.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HYO64_RS16075 rlmN 3349392..3350624 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  HYO64_RS16080 ndk 3350747..3351178 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  HYO64_RS16085 iscX 3351292..3351492 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  HYO64_RS16090 - 3351592..3352143 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  HYO64_RS16095 - 3352168..3353466 (-) 1299 WP_114155366.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30123.48 Da        Isoelectric Point: 9.3928

>NTDB_id=465075 HYO64_RS16070 WP_213067660.1 3348576..3349376(-) (pilF) [Acinetobacter baumannii strain M164-3]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=465075 HYO64_RS16070 WP_213067660.1 3348576..3349376(-) (pilF) [Acinetobacter baumannii strain M164-3]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCCGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAACGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726