Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   JQM61_RS31140 Genome accession   NZ_CP069198
Coordinates   6673079..6673543 (+) Length   154 a.a.
NCBI ID   WP_023129290.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 152962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6668079..6678543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQM61_RS31115 (JQM61_31115) yacG 6668249..6668449 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  JQM61_RS31120 (JQM61_31120) coaE 6668446..6669057 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  JQM61_RS31125 (JQM61_31125) pilD 6669054..6669926 (-) 873 WP_034037193.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  JQM61_RS31130 (JQM61_31130) pilC 6669927..6671144 (-) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  JQM61_RS31135 (JQM61_31135) pilB 6671148..6672848 (-) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JQM61_RS31140 (JQM61_31140) pilA/pilAI 6673079..6673543 (+) 465 WP_023129290.1 pilin Machinery gene
  JQM61_RS31145 (JQM61_31145) - 6673701..6675014 (+) 1314 WP_015648064.1 O-antigen ligase family protein -
  JQM61_RS31155 (JQM61_31155) nadC 6675195..6676043 (-) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16148.47 Da        Isoelectric Point: 8.9986

>NTDB_id=464453 JQM61_RS31140 WP_023129290.1 6673079..6673543(+) (pilA/pilAI) [Pseudomonas aeruginosa strain 152962]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=464453 JQM61_RS31140 WP_023129290.1 6673079..6673543(+) (pilA/pilAI) [Pseudomonas aeruginosa strain 152962]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364