Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HYE74_RS00540 Genome accession   NZ_CP058594
Coordinates   107250..108620 (+) Length   456 a.a.
NCBI ID   WP_014096526.1    Uniprot ID   A0A133KUJ2
Organism   Heyndrickxia coagulans strain ASRS217     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 102250..113620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYE74_RS00520 - 102457..102918 (+) 462 WP_013858048.1 CtsR family transcriptional regulator -
  HYE74_RS00525 - 102938..103483 (+) 546 WP_179234122.1 UvrB/UvrC motif-containing protein -
  HYE74_RS00530 - 103483..104550 (+) 1068 WP_014096529.1 protein arginine kinase -
  HYE74_RS00535 clpC 104567..107017 (+) 2451 WP_014096528.1 ATP-dependent protease ATP-binding subunit ClpC -
  HYE74_RS00540 radA 107250..108620 (+) 1371 WP_014096526.1 DNA repair protein RadA Machinery gene
  HYE74_RS00545 - 108967..110052 (+) 1086 WP_014096525.1 PIN/TRAM domain-containing protein -
  HYE74_RS00550 ispD 110119..110799 (+) 681 WP_014096524.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HYE74_RS00555 ispF 110802..111278 (+) 477 WP_014096523.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  HYE74_RS00560 gltX 111671..113131 (+) 1461 WP_013858056.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49792.20 Da        Isoelectric Point: 7.1420

>NTDB_id=464449 HYE74_RS00540 WP_014096526.1 107250..108620(+) (radA) [Heyndrickxia coagulans strain ASRS217]
MAKKTVKFVCQNCGYESPKWMGKCPQCSQWNQMVEEAETPKRRGFFLHPEPGNETKAAPITSIESKEEKRTKVDFGELNR
VLGGGIVAGSLVLIGGDPGIGKSTLLLQVSSQLAKHGLNVLYISGEESAKQTKLRADRLNIHTDHLYVLPETDLSVIQQA
IDELNPGFVVIDSIQTIYHPEIQSAPGSVSQVRETTAQLMRTAKTKNVAIFIVGHVTKEGSIAGPRVLEHMVDTVLYFEG
ERHNAYRILRAVKNRFGSTNEIAILEMKESGLEEVQNPSEIFLEERTKGAAGSTVVASIEGTRPVLVEIQALLAPTFFNM
PRRMASGIDYNRVTLIMAVLEKRAGLLIQNQDAYLKVAGGLKVDEPAVDLAVAVSVASSFRDKPTNADDCIIGEVGLTGE
VRRVSRIEQRVQEAAKLGFRRAIIPKNNMGGWTPPKEIQVIGVSTIQEALEAALGG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=464449 HYE74_RS00540 WP_014096526.1 107250..108620(+) (radA) [Heyndrickxia coagulans strain ASRS217]
ATGGCAAAAAAAACCGTAAAGTTTGTTTGCCAGAACTGCGGCTACGAGTCGCCAAAATGGATGGGAAAGTGCCCGCAATG
CAGCCAGTGGAACCAGATGGTGGAGGAAGCGGAGACGCCAAAACGGAGGGGCTTTTTTTTACATCCGGAGCCTGGAAATG
AAACCAAAGCGGCACCTATTACGTCTATAGAGTCTAAAGAAGAAAAAAGGACAAAGGTGGATTTTGGAGAGCTGAACCGT
GTCCTCGGCGGCGGCATTGTTGCCGGATCACTTGTTTTGATCGGCGGGGATCCCGGGATCGGCAAATCAACGCTGCTTTT
GCAAGTGTCATCACAACTGGCAAAGCACGGGTTAAATGTACTGTATATTTCCGGGGAGGAATCGGCGAAACAGACGAAGT
TAAGGGCGGACCGGTTAAACATCCATACCGACCATCTTTATGTGCTGCCGGAAACGGACCTTTCCGTGATCCAGCAGGCG
ATAGATGAACTGAATCCCGGCTTTGTGGTCATTGATTCCATCCAGACGATTTACCATCCGGAAATCCAGTCTGCGCCGGG
CAGTGTGTCGCAAGTGAGGGAAACGACTGCACAGCTGATGCGGACGGCAAAAACAAAAAACGTGGCCATCTTTATTGTCG
GCCACGTGACAAAAGAAGGCTCCATTGCCGGCCCACGTGTGCTTGAACATATGGTGGATACGGTCCTGTATTTTGAAGGT
GAACGCCATAACGCCTACCGGATATTAAGGGCGGTCAAAAACCGCTTTGGTTCGACGAATGAAATTGCGATTTTGGAAAT
GAAAGAATCCGGTTTGGAAGAAGTCCAGAACCCTTCCGAAATTTTTCTGGAAGAACGGACAAAAGGTGCAGCCGGTTCTA
CTGTGGTGGCTTCCATTGAGGGGACGCGCCCGGTCCTTGTCGAGATCCAGGCGCTGTTGGCCCCGACGTTTTTCAATATG
CCGCGGCGGATGGCATCCGGGATTGACTATAACCGTGTCACGCTGATTATGGCGGTGCTTGAAAAACGGGCCGGTCTTTT
GATTCAGAACCAGGATGCTTATTTAAAAGTCGCCGGCGGCCTGAAAGTGGATGAACCGGCAGTGGATTTGGCAGTTGCTG
TCAGCGTGGCATCCAGTTTTCGCGACAAGCCGACAAATGCGGATGACTGCATTATCGGAGAGGTCGGGCTGACGGGGGAA
GTCAGGCGGGTGTCCAGGATTGAGCAGCGCGTCCAGGAAGCGGCAAAACTTGGTTTCCGGCGGGCGATCATCCCGAAAAA
TAATATGGGCGGGTGGACGCCGCCAAAAGAGATTCAAGTGATCGGCGTCTCCACAATCCAGGAAGCTTTGGAAGCGGCAC
TCGGCGGATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(361-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133KUJ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

71.024

100

0.715
  radA Streptococcus mitis NCTC 12261

62.971

98.904

0.623
  radA Streptococcus mitis SK321

62.749

98.904

0.621
  radA Streptococcus pneumoniae Rx1

62.749

98.904

0.621
  radA Streptococcus pneumoniae D39

62.749

98.904

0.621
  radA Streptococcus pneumoniae R6

62.749

98.904

0.621
  radA Streptococcus pneumoniae TIGR4

62.749

98.904

0.621