Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   JNE32_RS08380 Genome accession   NZ_CP068982
Coordinates   1604234..1606180 (+) Length   648 a.a.
NCBI ID   WP_003232062.1    Uniprot ID   O34507
Organism   Bacillus subtilis strain pb2441     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 1599234..1611180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNE32_RS08355 (JNE32_08310) def 1599604..1600086 (+) 483 WP_015383630.1 peptide deformylase -
  JNE32_RS08360 (JNE32_08315) fmt 1600091..1601044 (+) 954 WP_029317789.1 methionyl-tRNA formyltransferase -
  JNE32_RS08365 (JNE32_08320) rsmB 1601031..1602374 (+) 1344 WP_250354608.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  JNE32_RS08370 (JNE32_08325) rlmN 1602378..1603469 (+) 1092 WP_014476780.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  JNE32_RS08375 (JNE32_08330) prpC 1603476..1604240 (+) 765 WP_003232064.1 protein-serine/threonine phosphatase PrpC -
  JNE32_RS08380 (JNE32_08335) stkP 1604234..1606180 (+) 1947 WP_003232062.1 serine/threonine protein kinase PrkC Regulator
  JNE32_RS08385 (JNE32_08340) rsgA 1606195..1607091 (+) 897 WP_014476783.1 ribosome small subunit-dependent GTPase A -
  JNE32_RS08390 (JNE32_08345) rpe 1607096..1607749 (+) 654 WP_029726492.1 ribulose-phosphate 3-epimerase -
  JNE32_RS08395 (JNE32_08350) thiN 1607822..1608466 (+) 645 WP_003245470.1 thiamine diphosphokinase -
  JNE32_RS08400 (JNE32_08355) spoVM 1608538..1608618 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  JNE32_RS08405 (JNE32_08360) rpmB 1608691..1608879 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  JNE32_RS08410 (JNE32_08365) yloU 1609156..1609518 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71866.49 Da        Isoelectric Point: 4.7568

>NTDB_id=464002 JNE32_RS08380 WP_003232062.1 1604234..1606180(+) (stkP) [Bacillus subtilis strain pb2441]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFNHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=464002 JNE32_RS08380 WP_003232062.1 1604234..1606180(+) (stkP) [Bacillus subtilis strain pb2441]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTCATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GACGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTATGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATTGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAAAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTTGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGATCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGCACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAAGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAATCATG
TAACAGTAAATGAAGTGAATGACGAGAAAAATGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTGGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTACAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGGAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCGAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(430-491)

PASTA domain (PASTA)

(498-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34507

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.555

89.043

0.361