Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CQZ96_RS16290 Genome accession   NZ_CP058272
Coordinates   3478761..3479564 (+) Length   267 a.a.
NCBI ID   WP_004725881.1    Uniprot ID   A0A6A1RK51
Organism   Acinetobacter sp. MYb10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3473761..3484564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CQZ96_RS16265 (CQZ96_016295) - 3474409..3475695 (+) 1287 WP_096735176.1 glycosyltransferase family 4 protein -
  CQZ96_RS16270 (CQZ96_016300) - 3475727..3476278 (+) 552 WP_004725875.1 phosphatase PAP2 family protein -
  CQZ96_RS16275 (CQZ96_016305) iscX 3476497..3476697 (+) 201 WP_105714393.1 Fe-S cluster assembly protein IscX -
  CQZ96_RS16280 (CQZ96_016310) ndk 3476841..3477272 (+) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  CQZ96_RS16285 (CQZ96_016315) rlmN 3477451..3478695 (+) 1245 WP_105714392.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CQZ96_RS16290 (CQZ96_016320) pilF 3478761..3479564 (+) 804 WP_004725881.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CQZ96_RS16295 (CQZ96_016325) - 3479555..3480343 (+) 789 WP_004725882.1 helix-turn-helix domain-containing protein -
  CQZ96_RS16300 (CQZ96_016330) ispG 3480365..3481480 (+) 1116 WP_004725885.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CQZ96_RS16305 (CQZ96_016335) hisS 3481477..3482772 (+) 1296 WP_034612341.1 histidine--tRNA ligase -
  CQZ96_RS16310 (CQZ96_016340) - 3482803..3483504 (+) 702 WP_004816992.1 YfgM family protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29946.10 Da        Isoelectric Point: 9.2123

>NTDB_id=463375 CQZ96_RS16290 WP_004725881.1 3478761..3479564(+) (pilF) [Acinetobacter sp. MYb10]
MYQKAVVILAIGLSALVVQGCQTTNSDTIIKKDPEKAVKVRTQLAAEYIRTGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDSKNAQARNNYGTYLFVTERYNDAIEQLSIAGAALGYDQRYKALENLGRVYLKVGD
VANAEKAFKQALQVNRDSIISLVELSEIFYLQQKSAQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=463375 CQZ96_RS16290 WP_004725881.1 3478761..3479564(+) (pilF) [Acinetobacter sp. MYb10]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTCGTACAGGGTTGTCAAACGACAAATTCAGA
CACAATAATAAAAAAAGATCCTGAAAAAGCCGTTAAAGTACGTACTCAGCTTGCTGCTGAATATATTCGTACAGGTGATT
TAGATGCTGCAAAACGCGCACTAGACCAAGCTTTAGAAACTGATTCACGTGATTCTGCGGCAAATATGATGATGGGTGTC
TTACTGCAACAAGAAGGCAGTAAGATCAGTATGGAAAAAGCAGACGGCTATTTTAAGCGTGCAATTTCCAGTGACTCAAA
AAATGCACAAGCTCGTAATAATTATGGAACATATTTATTTGTTACTGAGCGCTATAATGACGCGATTGAACAATTGAGTA
TAGCTGGTGCTGCGCTTGGTTATGATCAGCGTTACAAGGCTTTAGAAAATTTAGGTCGGGTTTACTTAAAAGTCGGTGAT
GTGGCAAATGCTGAAAAAGCATTCAAACAAGCTTTACAAGTCAATCGCGATTCGATTATTTCTCTGGTAGAGTTATCAGA
AATTTTCTATTTGCAACAAAAAAGTGCGCAAGCAAGTCAACTCTATGAGCAATATGTTCGTTTAGTAGGTCAGGACAAAC
AGGGTGCGAGAGCGCTTTGGATTGGTTTACGTATTGCACGTGCCAATAATGATCAGATGGGAATGCAAGTGTTGGTCAAT
CAACTGCGTGCATTGTTCCCGGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(146-177)

Tetratricopeptide repeat (TPR_8)

(40-69)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A1RK51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.717

99.251

0.742
  pilF Acinetobacter baylyi ADP1

67.843

95.506

0.648