Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HW576_RS22905 Genome accession   NZ_CP058262
Coordinates   4420593..4421645 (-) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain BIM B-1314D     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4415593..4426645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW576_RS22865 (HW576_22865) - 4416608..4417432 (+) 825 WP_013059169.1 YqhG family protein -
  HW576_RS22870 (HW576_22870) - 4417457..4417642 (-) 186 WP_013059170.1 YqzE family protein -
  HW576_RS22875 (HW576_22875) comGG 4417686..4418060 (-) 375 WP_013059171.1 competence type IV pilus minor pilin ComGG -
  HW576_RS22880 (HW576_22880) comGF 4418050..4418487 (-) 438 WP_053487735.1 competence type IV pilus minor pilin ComGF -
  HW576_RS22885 (HW576_22885) - 4418484..4418822 (-) 339 WP_013059173.1 type II secretion system protein -
  HW576_RS22890 (HW576_22890) comGD 4418806..4419243 (-) 438 WP_014458229.1 competence type IV pilus minor pilin ComGD -
  HW576_RS22895 (HW576_22895) comGC 4419230..4419541 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  HW576_RS22900 (HW576_22900) comGB 4419556..4420602 (-) 1047 WP_013059176.1 competence type IV pilus assembly protein ComGB -
  HW576_RS22905 (HW576_22905) comGA 4420593..4421645 (-) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  HW576_RS22910 (HW576_22910) - 4421915..4422298 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  HW576_RS22915 (HW576_22915) - 4422560..4422796 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  HW576_RS22920 (HW576_22920) - 4422834..4423466 (-) 633 WP_014458225.1 MBL fold metallo-hydrolase -
  HW576_RS22925 (HW576_22925) - 4423675..4423851 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  HW576_RS22930 (HW576_22930) - 4423887..4424789 (-) 903 WP_029320197.1 M14 family metallocarboxypeptidase -
  HW576_RS22935 (HW576_22935) - 4424901..4425869 (-) 969 WP_013059183.1 ROK family glucokinase -
  HW576_RS22940 (HW576_22940) - 4425872..4426078 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=463320 HW576_RS22905 WP_013059177.1 4420593..4421645(-) (comGA) [Priestia megaterium strain BIM B-1314D]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=463320 HW576_RS22905 WP_013059177.1 4420593..4421645(-) (comGA) [Priestia megaterium strain BIM B-1314D]
TTGACCGAAATTGAACAGTTTGCAACGTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACCCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACACACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACGGGTCCTACAGGGTCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGGGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGAA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGTGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTGATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586