Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HW369_RS27600 Genome accession   NZ_CP058255
Coordinates   5301445..5302821 (-) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia megaterium strain BHS1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5296445..5307821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW369_RS27580 ispF 5297914..5298393 (-) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  HW369_RS27585 ispD 5298390..5299091 (-) 702 WP_025753186.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HW369_RS27590 - 5299117..5300214 (-) 1098 WP_013054901.1 PIN/TRAM domain-containing protein -
  HW369_RS27595 disA 5300362..5301441 (-) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  HW369_RS27600 radA 5301445..5302821 (-) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  HW369_RS27605 clpC 5302945..5305386 (-) 2442 WP_014462041.1 ATP-dependent protease ATP-binding subunit ClpC -
  HW369_RS27610 - 5305407..5306480 (-) 1074 WP_195695612.1 protein arginine kinase -
  HW369_RS27615 - 5306484..5307026 (-) 543 WP_025753183.1 UvrB/UvrC motif-containing protein -
  HW369_RS27620 - 5307068..5307529 (-) 462 WP_013054895.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=463155 HW369_RS27600 WP_013054899.1 5301445..5302821(-) (radA) [Priestia megaterium strain BHS1]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=463155 HW369_RS27600 WP_013054899.1 5301445..5302821(-) (radA) [Priestia megaterium strain BHS1]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCAGCAAAATGGATGGGGAAATGTCCCGGATG
CGGAAGCTGGAATTCGATGGTAGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTCCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGAATTTTTACACCTTCTGCAGAGTTA
AATCGTGTATTAGGAGGAGGTATTGTCAGAGGGTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCCGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAGCAAA
CGAAGTTAAGAGCGGACAGGCTAGGTGTAAAAGCTGAAGAGCTTTATGTTCATGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGCATGCAGCCTGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTCACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACGAATGGAATTGCCATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCAGGACCAAGGCTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGCCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGATTTGGATCCACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGGTCTCAGGGAGCAGCAG
GATCGGTTGTTGTTGCTTCAATGGAAGGGACAAGACCCGTCTTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACGGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTAAAATTAGATGAACCAGCAATTGATTTAGCTG
TAGCCGTTAGTATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGTGAAGTACGCCGAGTATCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATCCC
AGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624