Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HW369_RS04040 Genome accession   NZ_CP058255
Coordinates   780466..781518 (+) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain BHS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 775466..786518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW369_RS04005 - 776033..776239 (+) 207 WP_195695901.1 YqgQ family protein -
  HW369_RS04010 - 776242..777210 (+) 969 WP_013059183.1 ROK family glucokinase -
  HW369_RS04015 - 777322..778224 (+) 903 WP_195695902.1 M14 family metallocarboxypeptidase -
  HW369_RS04020 - 778260..778436 (-) 177 WP_013059181.1 DUF2759 domain-containing protein -
  HW369_RS04025 - 778645..779277 (+) 633 WP_195695903.1 MBL fold metallo-hydrolase -
  HW369_RS04030 - 779315..779551 (-) 237 WP_013059179.1 DUF2626 domain-containing protein -
  HW369_RS04035 - 779813..780196 (+) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  HW369_RS04040 comGA 780466..781518 (+) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  HW369_RS04045 comGB 781509..782555 (+) 1047 WP_028410643.1 competence type IV pilus assembly protein ComGB -
  HW369_RS04050 comGC 782570..782881 (+) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  HW369_RS04055 comGD 782823..783305 (+) 483 WP_244658419.1 competence type IV pilus minor pilin ComGD -
  HW369_RS04060 - 783289..783627 (+) 339 WP_195695904.1 type II secretion system protein -
  HW369_RS04065 comGF 783624..784061 (+) 438 WP_195695905.1 competence type IV pilus minor pilin ComGF -
  HW369_RS04070 comGG 784051..784425 (+) 375 WP_195695906.1 competence type IV pilus minor pilin ComGG -
  HW369_RS04075 - 784469..784654 (+) 186 WP_013059170.1 YqzE family protein -
  HW369_RS04080 - 784679..785503 (-) 825 WP_195695907.1 YqhG family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=463123 HW369_RS04040 WP_013059177.1 780466..781518(+) (comGA) [Priestia megaterium strain BHS1]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=463123 HW369_RS04040 WP_013059177.1 780466..781518(+) (comGA) [Priestia megaterium strain BHS1]
TTGACCGAAATTGAACAGTTTGCGACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAACGAAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATACACTCTTTTACACGAA
GTAAAGCATCTTTTTAATCGAAATGTTATTACACTAGAAGACCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGGTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGCGTTAGTTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGCCCGCTGTGTTCCGGAAAATGCCATCCCGCTTGTAAAAAAATGAGAA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586