Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HWH77_RS12285 Genome accession   NZ_CP058216
Coordinates   2408811..2409248 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain LF01     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2403811..2414248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWH77_RS19770 - 2404390..2404515 (-) 126 Protein_2391 hypothetical protein -
  HWH77_RS12250 (HWH77_12255) - 2404668..2405870 (+) 1203 WP_069473143.1 GTP-binding protein -
  HWH77_RS12255 (HWH77_12260) - 2405935..2407071 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  HWH77_RS12260 (HWH77_12265) - 2407086..2407520 (+) 435 WP_003156592.1 RDD family protein -
  HWH77_RS12265 (HWH77_12270) - 2407637..2407912 (+) 276 WP_371745793.1 YckD family protein -
  HWH77_RS12270 (HWH77_12275) - 2408016..2408132 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  HWH77_RS12275 (HWH77_12280) - 2408131..2408350 (+) 220 Protein_2397 family 1 glycosylhydrolase -
  HWH77_RS12280 (HWH77_12285) nin/comJ 2408391..2408789 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  HWH77_RS12285 (HWH77_12290) nucA/comI 2408811..2409248 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HWH77_RS12290 (HWH77_12295) hxlB 2409607..2410164 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  HWH77_RS12295 (HWH77_12300) hxlA 2410161..2410796 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  HWH77_RS12300 (HWH77_12305) - 2411028..2411390 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=462718 HWH77_RS12285 WP_003156588.1 2408811..2409248(-) (nucA/comI) [Bacillus velezensis strain LF01]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=462718 HWH77_RS12285 WP_003156588.1 2408811..2409248(-) (nucA/comI) [Bacillus velezensis strain LF01]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641