Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JNN40_RS17330 Genome accession   NZ_CP068802
Coordinates   3651314..3651991 (-) Length   225 a.a.
NCBI ID   WP_001241107.1    Uniprot ID   -
Organism   Escherichia coli strain RIVM_C018249     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3646314..3656991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNN40_RS17315 (JNN40_17315) chiA 3647672..3650374 (+) 2703 WP_000773171.1 bifunctional chitinase/lysozyme -
  JNN40_RS17320 (JNN40_17320) bfd 3650543..3650737 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JNN40_RS17325 (JNN40_17325) bfr 3650809..3651285 (+) 477 WP_000675504.1 bacterioferritin -
  JNN40_RS17330 (JNN40_17330) pilD 3651314..3651991 (-) 678 WP_001241107.1 prepilin peptidase GspO Machinery gene
  JNN40_RS17335 (JNN40_17335) gspM 3651991..3652452 (-) 462 WP_001389811.1 type II secretion system protein GspM -
  JNN40_RS17340 (JNN40_17340) gspL 3652449..3653612 (-) 1164 WP_001115232.1 type II secretion system protein GspL -
  JNN40_RS17345 (JNN40_17345) gspK 3653627..3654610 (-) 984 WP_001058526.1 type II secretion system minor pseudopilin GspK -
  JNN40_RS17350 (JNN40_17350) gspJ 3654603..3655190 (-) 588 WP_000610627.1 type II secretion system minor pseudopilin GspJ -
  JNN40_RS17355 (JNN40_17355) gspI 3655183..3655560 (-) 378 WP_001041753.1 type II secretion system minor pseudopilin GspI -
  JNN40_RS17360 (JNN40_17360) gspH 3655557..3656066 (-) 510 WP_001076042.1 type II secretion system minor pseudopilin GspH -
  JNN40_RS17365 (JNN40_17365) gspG 3656074..3656511 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24914.85 Da        Isoelectric Point: 7.9563

>NTDB_id=461538 JNN40_RS17330 WP_001241107.1 3651314..3651991(-) (pilD) [Escherichia coli strain RIVM_C018249]
MAMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACVLAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=461538 JNN40_RS17330 WP_001241107.1 3651314..3651991(-) (pilD) [Escherichia coli strain RIVM_C018249]
ATGGCAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGTCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTGCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.463

100

0.382