Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JNN46_RS01920 Genome accession   NZ_CP068799
Coordinates   423955..424632 (-) Length   225 a.a.
NCBI ID   WP_000178177.1    Uniprot ID   -
Organism   Escherichia coli strain RIVM_C017887     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418955..429632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNN46_RS01905 (JNN46_01905) chiA 420313..423015 (+) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -
  JNN46_RS01910 (JNN46_01910) bfd 423184..423378 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JNN46_RS01915 (JNN46_01915) bfr 423450..423926 (+) 477 WP_000675504.1 bacterioferritin -
  JNN46_RS01920 (JNN46_01920) pilD 423955..424632 (-) 678 WP_000178177.1 prepilin peptidase GspO Machinery gene
  JNN46_RS01925 (JNN46_01925) gspM 424632..425093 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  JNN46_RS01930 (JNN46_01930) gspL 425090..426253 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  JNN46_RS01935 (JNN46_01935) gspK 426268..427251 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  JNN46_RS01940 (JNN46_01940) gspJ 427244..427831 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  JNN46_RS01945 (JNN46_01945) gspI 427824..428219 (-) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  JNN46_RS01950 (JNN46_01950) gspH 428216..428725 (-) 510 WP_001076037.1 type II secretion system minor pseudopilin GspH -
  JNN46_RS01955 (JNN46_01955) gspG 428733..429170 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24900.77 Da        Isoelectric Point: 7.6364

>NTDB_id=461233 JNN46_RS01920 WP_000178177.1 423955..424632(-) (pilD) [Escherichia coli strain RIVM_C017887]
MTMLLPLFILVGFIASYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=461233 JNN46_RS01920 WP_000178177.1 423955..424632(-) (pilD) [Escherichia coli strain RIVM_C017887]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAAGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382