Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JNN54_RS02445 Genome accession   NZ_CP068795
Coordinates   519898..520575 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain RIVM_C011532     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514898..525575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNN54_RS02430 (JNN54_02430) chiA 516265..518958 (+) 2694 WP_072649929.1 bifunctional chitinase/lysozyme -
  JNN54_RS02435 (JNN54_02435) bfd 519127..519321 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JNN54_RS02440 (JNN54_02440) bfr 519393..519869 (+) 477 WP_000675504.1 bacterioferritin -
  JNN54_RS02445 (JNN54_02445) pilD 519898..520575 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JNN54_RS02450 (JNN54_02450) gspM 520575..521036 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JNN54_RS02455 (JNN54_02455) gspL 521033..522196 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JNN54_RS02460 (JNN54_02460) gspK 522211..523194 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JNN54_RS02465 (JNN54_02465) gspJ 523187..523774 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JNN54_RS02470 (JNN54_02470) gspI 523767..524144 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JNN54_RS02475 (JNN54_02475) gspH 524141..524650 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  JNN54_RS02480 (JNN54_02480) gspG 524658..525095 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=461068 JNN54_RS02445 WP_000178161.1 519898..520575(-) (pilD) [Escherichia coli strain RIVM_C011532]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=461068 JNN54_RS02445 WP_000178161.1 519898..520575(-) (pilD) [Escherichia coli strain RIVM_C011532]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378