Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JKV47_RS07305 Genome accession   NZ_CP068680
Coordinates   1547089..1547766 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain NCCP12480     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1542089..1552766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKV47_RS07280 (JKV47_07205) - 1542679..1542930 (+) 252 Protein_1417 hypothetical protein -
  JKV47_RS07285 (JKV47_07210) - 1542967..1543664 (+) 698 WP_103215986.1 IS1-like element IS1A family transposase -
  JKV47_RS07290 (JKV47_07215) chiA 1543657..1546149 (+) 2493 WP_235144922.1 bifunctional chitinase/lysozyme -
  JKV47_RS07295 (JKV47_07220) bfd 1546318..1546512 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JKV47_RS07300 (JKV47_07225) bfr 1546584..1547060 (+) 477 WP_000675504.1 bacterioferritin -
  JKV47_RS07305 (JKV47_07230) pilD 1547089..1547766 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JKV47_RS07310 (JKV47_07235) gspM 1547766..1548227 (-) 462 WP_115194741.1 type II secretion system protein GspM -
  JKV47_RS07315 (JKV47_07240) gspL 1548224..1549387 (-) 1164 WP_115194742.1 type II secretion system protein GspL -
  JKV47_RS07320 (JKV47_07245) gspK 1549402..1550385 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  JKV47_RS07325 (JKV47_07250) gspJ 1550378..1550965 (-) 588 WP_000610366.1 type II secretion system minor pseudopilin GspJ -
  JKV47_RS07330 (JKV47_07255) gspI 1550958..1551335 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JKV47_RS07335 (JKV47_07260) gspH 1551332..1551841 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  JKV47_RS07340 (JKV47_07265) gspG 1551849..1552286 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=460616 JKV47_RS07305 WP_000178161.1 1547089..1547766(-) (pilD) [Escherichia coli strain NCCP12480]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=460616 JKV47_RS07305 WP_000178161.1 1547089..1547766(-) (pilD) [Escherichia coli strain NCCP12480]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378