Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HV228_RS20375 Genome accession   NZ_CP056716
Coordinates   4150214..4151647 (+) Length   477 a.a.
NCBI ID   WP_181593933.1    Uniprot ID   -
Organism   Enterobacter asburiae strain RHBSTW-00516     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4145214..4156647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV228_RS20345 (HV228_20345) - 4145781..4146758 (+) 978 WP_181593932.1 calcium/sodium antiporter -
  HV228_RS20350 (HV228_20350) kdsD 4146772..4147758 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  HV228_RS20355 (HV228_20355) kdsC 4147773..4148339 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HV228_RS20360 (HV228_20360) lptC 4148336..4148911 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  HV228_RS20365 (HV228_20365) lptA 4148880..4149434 (+) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HV228_RS20370 (HV228_20370) lptB 4149441..4150166 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  HV228_RS20375 (HV228_20375) rpoN/rpoN1 4150214..4151647 (+) 1434 WP_181593933.1 RNA polymerase factor sigma-54 Machinery gene
  HV228_RS20380 (HV228_20380) hpf 4151670..4151957 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  HV228_RS20385 (HV228_20385) ptsN 4152040..4152531 (+) 492 WP_181593934.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HV228_RS20390 (HV228_20390) rapZ 4152577..4153431 (+) 855 WP_014885397.1 RNase adapter RapZ -
  HV228_RS20395 (HV228_20395) npr 4153428..4153700 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  HV228_RS20400 (HV228_20400) mtgA 4153740..4154465 (-) 726 WP_181593935.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HV228_RS20405 (HV228_20405) elbB 4154462..4155115 (-) 654 WP_181595226.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54183.92 Da        Isoelectric Point: 4.3149

>NTDB_id=460309 HV228_RS20375 WP_181593933.1 4150214..4151647(+) (rpoN/rpoN1) [Enterobacter asburiae strain RHBSTW-00516]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEIDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=460309 HV228_RS20375 WP_181593933.1 4150214..4151647(+) (rpoN/rpoN1) [Enterobacter asburiae strain RHBSTW-00516]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGATAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTACCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGTGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGG
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAACGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGTTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48