Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HV254_RS02175 Genome accession   NZ_CP056676
Coordinates   464390..465823 (-) Length   477 a.a.
NCBI ID   WP_045622108.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain RHBSTW-00642     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459390..470823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV254_RS02145 (HV254_02145) elbB 460922..461575 (+) 654 WP_038416794.1 isoprenoid biosynthesis glyoxalase ElbB -
  HV254_RS02150 (HV254_02150) mtgA 461572..462297 (+) 726 WP_022649531.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HV254_RS02155 (HV254_02155) npr 462337..462609 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  HV254_RS02160 (HV254_02160) rapZ 462606..463460 (-) 855 WP_003861870.1 RNase adapter RapZ -
  HV254_RS02165 (HV254_02165) ptsN 463506..463997 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HV254_RS02170 (HV254_02170) hpf 464080..464367 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  HV254_RS02175 (HV254_02175) rpoN/rpoN1 464390..465823 (-) 1434 WP_045622108.1 RNA polymerase factor sigma-54 Machinery gene
  HV254_RS02180 (HV254_02180) lptB 465871..466596 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  HV254_RS02185 (HV254_02185) lptA 466603..467157 (-) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HV254_RS02190 (HV254_02190) lptC 467126..467701 (-) 576 WP_022649529.1 LPS export ABC transporter periplasmic protein LptC -
  HV254_RS02195 (HV254_02195) kdsC 467698..468264 (-) 567 WP_045622106.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HV254_RS02200 (HV254_02200) kdsD 468279..469265 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  HV254_RS02205 (HV254_02205) - 469279..470256 (-) 978 WP_045622105.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54127.03 Da        Isoelectric Point: 4.3575

>NTDB_id=460141 HV254_RS02175 WP_045622108.1 464390..465823(-) (rpoN/rpoN1) [Enterobacter hormaechei strain RHBSTW-00642]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISEHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVQMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=460141 HV254_RS02175 WP_045622108.1 464390..465823(-) (rpoN/rpoN1) [Enterobacter hormaechei strain RHBSTW-00642]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAACAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCTCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAG
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATTGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGAGCATCTGGATTTGCTGGCAAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTCAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAATCGATCCAGACCAG
CGAACCGGAATACGTTATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAAAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAATTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCCAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGCCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48