Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CBF_RS12760 Genome accession   NC_017297
Coordinates   2725511..2726563 (-) Length   350 a.a.
NCBI ID   WP_012100441.1    Uniprot ID   A7GGC6
Organism   Clostridium botulinum F str. 230613     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2720511..2731563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBF_RS12740 (CBF_2589) ftsZ 2721039..2722148 (-) 1110 WP_003386373.1 cell division protein FtsZ -
  CBF_RS12745 (CBF_2590) ftsA 2722171..2723424 (-) 1254 WP_003386375.1 cell division protein FtsA -
  CBF_RS12750 (CBF_2591) - 2723581..2724855 (-) 1275 WP_012100439.1 glycosyltransferase family 2 protein -
  CBF_RS12755 (CBF_2592) - 2725293..2725436 (+) 144 WP_012100440.1 hypothetical protein -
  CBF_RS12760 (CBF_2593) pilT 2725511..2726563 (-) 1053 WP_012100441.1 type IV pilus twitching motility protein PilT Machinery gene
  CBF_RS12765 (CBF_2594) aroE 2726565..2727341 (-) 777 WP_012100442.1 shikimate dehydrogenase -
  CBF_RS12770 (CBF_2595) sigK 2727480..2728184 (-) 705 WP_004440491.1 RNA polymerase sporulation sigma factor SigK -
  CBF_RS12775 (CBF_2596) - 2728610..2728789 (-) 180 WP_003386384.1 aspartyl-phosphate phosphatase Spo0E family protein -
  CBF_RS19505 (CBF_2597) - 2729234..2729371 (-) 138 WP_004452043.1 hypothetical protein -
  CBF_RS12780 - 2729762..2730016 (+) 255 WP_003386388.1 hypothetical protein -
  CBF_RS21000 - 2730223..2730522 (-) 300 Protein_2500 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39024.08 Da        Isoelectric Point: 8.0876

>NTDB_id=45996 CBF_RS12760 WP_012100441.1 2725511..2726563(-) (pilT) [Clostridium botulinum F str. 230613]
MKQLKELLEKTINFGASDLHLSVGVEPTIRINGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVATNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=45996 CBF_RS12760 WP_012100441.1 2725511..2726563(-) (pilT) [Clostridium botulinum F str. 230613]
TTGAAACAATTAAAAGAATTATTAGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCCGTAGGAGTAGAACC
AACTATAAGAATAAATGGACAATTAAGTAAAATATTTAAAGAAAAACTTACACCAGAAGATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCCTATTCTATATCGGGCTTAGGTAGA
TTTAGAGTAAGCGTGTTTAAACAAAGAGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTAAAGATTCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGTAAAAGCACTACCTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCCTTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGGCATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAGCAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAGCTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTACGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GGC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Pseudomonas aeruginosa PAK

49.405

96

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

94.286

0.474
  pilT Vibrio cholerae strain A1552

50.303

94.286

0.474
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.81

96

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.552

99.429

0.463
  pilT Neisseria meningitidis 8013

47.761

95.714

0.457
  pilT Neisseria gonorrhoeae MS11

47.761

95.714

0.457
  pilU Acinetobacter baylyi ADP1

41.124

96.571

0.397
  pilU Pseudomonas stutzeri DSM 10701

41.194

95.714

0.394
  pilU Vibrio cholerae strain A1552

38.643

96.857

0.374

Multiple sequence alignment