Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JK368_RS18230 Genome accession   NZ_CP068394
Coordinates   3770551..3771228 (+) Length   225 a.a.
NCBI ID   WP_000178166.1    Uniprot ID   -
Organism   Escherichia coli strain M-17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3765551..3776228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JK368_RS18195 (JK368_18195) gspG 3766031..3766468 (+) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -
  JK368_RS18200 (JK368_18200) gspH 3766476..3766985 (+) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  JK368_RS18205 (JK368_18205) gspI 3766982..3767359 (+) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  JK368_RS18210 (JK368_18210) gspJ 3767352..3767939 (+) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  JK368_RS18215 (JK368_18215) gspK 3767932..3768915 (+) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -
  JK368_RS18220 (JK368_18220) gspL 3768930..3770093 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  JK368_RS18225 (JK368_18225) gspM 3770090..3770551 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  JK368_RS18230 (JK368_18230) pilD 3770551..3771228 (+) 678 WP_000178166.1 prepilin peptidase GspO Machinery gene
  JK368_RS18235 (JK368_18235) bfr 3771257..3771733 (-) 477 WP_000675504.1 bacterioferritin -
  JK368_RS18240 (JK368_18240) bfd 3771805..3771999 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JK368_RS18245 (JK368_18245) chiA 3772168..3774870 (-) 2703 WP_000773168.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24814.64 Da        Isoelectric Point: 7.6364

>NTDB_id=459680 JK368_RS18230 WP_000178166.1 3770551..3771228(+) (pilD) [Escherichia coli strain M-17]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLAHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=459680 JK368_RS18230 WP_000178166.1 3770551..3771228(+) (pilD) [Escherichia coli strain M-17]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGCTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382