Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JI738_RS17420 Genome accession   NZ_CP068283
Coordinates   3522431..3523108 (+) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain CY598     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3517431..3528108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JI738_RS17385 (JI738_17315) gspG 3517911..3518348 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  JI738_RS17390 (JI738_17320) gspH 3518356..3518865 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  JI738_RS17395 (JI738_17325) gspI 3518862..3519239 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JI738_RS17400 (JI738_17330) gspJ 3519232..3519819 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JI738_RS17405 (JI738_17335) gspK 3519812..3520795 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JI738_RS17410 (JI738_17340) gspL 3520810..3521973 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  JI738_RS17415 (JI738_17345) gspM 3521970..3522431 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  JI738_RS17420 (JI738_17350) pilD 3522431..3523108 (+) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  JI738_RS17425 (JI738_17355) bfr 3523137..3523613 (-) 477 WP_000675504.1 bacterioferritin -
  JI738_RS17430 (JI738_17360) bfd 3523685..3523879 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JI738_RS17435 (JI738_17365) chiA 3524048..3526741 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=459599 JI738_RS17420 WP_000178154.1 3522431..3523108(+) (pilD) [Escherichia coli strain CY598]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=459599 JI738_RS17420 WP_000178154.1 3522431..3523108(+) (pilD) [Escherichia coli strain CY598]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378