Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JJW05_RS01915 Genome accession   NZ_CP068279
Coordinates   417534..418211 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain CY708     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412534..423211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJW05_RS01900 (JJW05_01875) chiA 413901..416594 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  JJW05_RS01905 (JJW05_01880) bfd 416763..416957 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JJW05_RS01910 (JJW05_01885) bfr 417029..417505 (+) 477 WP_000675504.1 bacterioferritin -
  JJW05_RS01915 (JJW05_01890) pilD 417534..418211 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  JJW05_RS01920 (JJW05_01895) gspM 418211..418672 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JJW05_RS01925 (JJW05_01900) gspL 418669..419832 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JJW05_RS01930 (JJW05_01905) gspK 419847..420830 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JJW05_RS01935 (JJW05_01910) gspJ 420823..421410 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JJW05_RS01940 (JJW05_01915) gspI 421403..421780 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JJW05_RS01945 (JJW05_01920) gspH 421777..422286 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  JJW05_RS01950 (JJW05_01925) gspG 422294..422731 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=459397 JJW05_RS01915 WP_000178154.1 417534..418211(-) (pilD) [Escherichia coli strain CY708]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=459397 JJW05_RS01915 WP_000178154.1 417534..418211(-) (pilD) [Escherichia coli strain CY708]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378