Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HWQ15_RS05395 Genome accession   NZ_CP056118
Coordinates   1133475..1134908 (+) Length   477 a.a.
NCBI ID   WP_022649530.1    Uniprot ID   A0AAI9D3Q5
Organism   Enterobacter sp. DSM 30060     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1128475..1139908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ15_RS05365 (HWQ15_05365) - 1129042..1130019 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  HWQ15_RS05370 (HWQ15_05370) kdsD 1130033..1131019 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  HWQ15_RS05375 (HWQ15_05375) kdsC 1131034..1131600 (+) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HWQ15_RS05380 (HWQ15_05380) lptC 1131597..1132172 (+) 576 WP_022649529.1 LPS export ABC transporter periplasmic protein LptC -
  HWQ15_RS05385 (HWQ15_05385) lptA 1132141..1132695 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HWQ15_RS05390 (HWQ15_05390) lptB 1132702..1133427 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  HWQ15_RS05395 (HWQ15_05395) rpoN/rpoN1 1133475..1134908 (+) 1434 WP_022649530.1 RNA polymerase factor sigma-54 Machinery gene
  HWQ15_RS05400 (HWQ15_05400) hpf 1134931..1135218 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  HWQ15_RS05405 (HWQ15_05405) ptsN 1135301..1135792 (+) 492 WP_063149061.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HWQ15_RS05410 (HWQ15_05410) rapZ 1135838..1136692 (+) 855 WP_003861870.1 RNase adapter RapZ -
  HWQ15_RS05415 (HWQ15_05415) npr 1136689..1136961 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  HWQ15_RS05420 (HWQ15_05420) mtgA 1137001..1137726 (-) 726 WP_045331541.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HWQ15_RS05425 (HWQ15_05425) elbB 1137723..1138376 (-) 654 WP_063149060.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54128.02 Da        Isoelectric Point: 4.3409

>NTDB_id=458169 HWQ15_RS05395 WP_022649530.1 1133475..1134908(+) (rpoN/rpoN1) [Enterobacter sp. DSM 30060]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISEHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=458169 HWQ15_RS05395 WP_022649530.1 1133475..1134908(+) (rpoN/rpoN1) [Enterobacter sp. DSM 30060]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAACAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCTCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAG
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGCACCCCTTCCGGGACGCGTGCAGATTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATAGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGAGCATCTGGATTTGCTGGCAAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTCAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAATCTATCCAGACCAG
CGAACCGGAATACGTTATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAAAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACAGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGCCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48