Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   HWQ56_RS02300 Genome accession   NZ_CP056030
Coordinates   477994..480213 (-) Length   739 a.a.
NCBI ID   WP_158154477.1    Uniprot ID   A0A7D5H365
Organism   Pseudomonas eucalypticola strain NP-1     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472994..485213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ56_RS02280 (HWQ56_02280) hslU 473280..474617 (-) 1338 WP_158156401.1 ATP-dependent protease ATPase subunit HslU -
  HWQ56_RS02285 (HWQ56_02285) hslV 474693..475223 (-) 531 WP_027979824.1 ATP-dependent protease subunit HslV -
  HWQ56_RS02290 (HWQ56_02290) - 475409..476098 (-) 690 WP_158154479.1 SPOR domain-containing protein -
  HWQ56_RS02295 (HWQ56_02295) argS 476100..477836 (-) 1737 WP_158154478.1 arginine--tRNA ligase -
  HWQ56_RS02300 (HWQ56_02300) priA 477994..480213 (-) 2220 WP_158154477.1 primosomal protein N' Machinery gene
  HWQ56_RS02305 (HWQ56_02305) rpmE 480417..480635 (+) 219 WP_158154476.1 50S ribosomal protein L31 -
  HWQ56_RS02310 (HWQ56_02310) - 480876..482144 (+) 1269 WP_158154475.1 malic enzyme-like NAD(P)-binding protein -
  HWQ56_RS02315 (HWQ56_02315) - 482206..484629 (-) 2424 WP_176569687.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81822.31 Da        Isoelectric Point: 8.4297

>NTDB_id=457950 HWQ56_RS02300 WP_158154477.1 477994..480213(-) (priA) [Pseudomonas eucalypticola strain NP-1]
MPDVILRLALPSPLRRLFDYRAPAGMPASAFTPGMRLRVPFGRREMIGILVEVADQSEVPADKLKPASALLDPVSPLPAP
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGELAEARQERFWLAAPGARQDDPRIKNAPRQREALAALAQHPHGVAHPLL
SKLGLNKTSLDLLLEKELVQVEVRKHAPVPRHEHWLAQPELPLNSEQRAAFEAVRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKHPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPILLGSATPSLESLHNAYTGRYGLLKMEHRAGGAKPPKFLRLDVKS
RPLDSGISGPMQQAIGQTLANGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARTTVHQRSGELRCHHCGHVERVPRQC
PKCNTVDLRPVGAGTERAEERLSILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACGVAEQLLEQLQLSGIELLGPVPAPMERRAGRYRAQLLLQASARAPLHKLIASWLLNLEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=457950 HWQ56_RS02300 WP_158154477.1 477994..480213(-) (priA) [Pseudomonas eucalypticola strain NP-1]
GTGCCCGACGTCATCCTGCGCCTCGCCCTGCCCTCGCCGCTGCGACGCCTGTTCGACTACCGGGCGCCCGCCGGCATGCC
GGCCAGCGCGTTCACCCCAGGCATGCGCCTGCGAGTACCGTTCGGCCGCCGGGAAATGATCGGCATCCTGGTGGAGGTCG
CCGACCAGAGCGAAGTGCCCGCCGACAAGCTCAAGCCCGCCAGCGCCTTGCTCGACCCGGTCAGCCCGTTGCCGGCGCCG
CTGTTCAAGCTGTGCCTGTGGACGGCGCAATACTATCAGCACAGCCTGGGCGACACGCTGAGCTGGGCTCTGCCAGTCCT
GTTGCGCCAGGGGGAACTGGCCGAGGCCCGACAGGAACGCTTCTGGCTGGCCGCGCCGGGCGCCCGTCAGGATGACCCGC
GTATCAAGAATGCGCCGCGTCAACGTGAAGCGCTGGCCGCACTGGCCCAGCACCCCCACGGCGTGGCCCACCCCTTGCTG
AGCAAGCTGGGCCTGAACAAGACCAGCCTCGACCTGCTGCTGGAAAAAGAGCTGGTCCAGGTAGAAGTGCGCAAGCACGC
GCCGGTACCACGCCACGAACACTGGCTGGCCCAGCCCGAGTTGCCGCTCAACAGCGAGCAGCGCGCCGCCTTCGAGGCCG
TGCGCGCTGGCTTCGACAGTTTCCACGCCTTCCTGCTCGCCGGGGTTACCGGCAGTGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGAGGCCGGCAAGCAGGCGTTGGTGCTGATCCCGGAGATCAATCTGGGCCCGCAGACCCTCGC
CCGTTTCGAACAGCGCTTCAACGCGCGCATTGCCCTGCTGCATTCCGCCGTCAATGACCGTGAGCGCCTGGATGCCTGGC
TGGCAGCCCGAGACGGCGAGGCAGATATCATCATCGGTACCCGCTCGGCATTATTCACGCCGATGAAACACCCCGGGCTG
ATCATCATCGACGAAGAGCACGACGGCTCCTATAAACAGCAGGAAGGCTTGCGCTATCACGCCCGAGACCTGGCGCTGGT
GCGCGCGCGGCAGGAGAACATCCCCATTCTGCTGGGTTCGGCGACGCCGTCACTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTACGGCCTGCTGAAAATGGAACACCGCGCCGGGGGTGCCAAACCGCCGAAATTCCTGCGCCTGGACGTCAAGAGC
CGGCCCCTGGACAGCGGCATCAGCGGGCCCATGCAGCAAGCCATCGGCCAGACCCTGGCCAACGGCCAACAGGTGCTGGT
GTTTCTCAACCGGCGCGGTTTCGCCCCTACCCTGCTGTGTCACGATTGCGGCTGGCTGTCCGAATGCCCACGCTGCGACG
CACGCACCACCGTGCACCAGCGCTCCGGCGAGTTGCGCTGCCACCACTGCGGGCACGTGGAGCGCGTGCCACGCCAGTGC
CCCAAGTGCAACACCGTGGACCTGCGCCCCGTGGGGGCCGGTACCGAACGGGCAGAAGAGCGCCTGAGTATCCTGTTTCC
CGATTACCCCGTGCTGCGGGTGGACCGGGACAGCACCTCGCGCAAAGACGCCATGAACCAGTTATTCGCCACCATTCAAA
AAGGGCAACCGTGCATTCTGGTGGGCACCCAGATGCTCGCCAAAGGCCATCATTTTCCGCGGGTCACCCTGGTGTCGATC
CTGGACGCGGACGGCGGCCTGTTTTCCGGTGACTTCCGCGCCAGCGAGCGCATGGCCCAGTTGATCGTGCAGGTAGCCGG
CCGCGCAGGCCGGGCAGAAGAGCCGGGCAAGGTGATTATCCAGACCCACCTGGCCGACCACCCGCTACTGGTGCAACTGA
CCGAACAAGGCTACTTCGCCTTCGCTGAACAGGCCCTGAGCGAGCGCCGCGCCGCCGGCCTGCCGCCGTTCGCCCACCTG
GCCCTGCTGCGCGCCGAAGCCCACAAGCCAGGCCAGGCCGAAGGCTTCCTCGACGAAGCCTGCGGCGTGGCCGAACAACT
GCTGGAACAATTGCAGCTTTCGGGCATCGAACTGCTCGGGCCGGTGCCAGCGCCCATGGAACGCCGTGCCGGCCGCTACC
GCGCACAGTTGCTGTTGCAAGCCAGCGCCAGGGCGCCACTGCACAAGCTGATCGCCAGCTGGCTGCTTAACCTGGAGCAG
ATGCCCAGCGGGCGCCAGGTGCGCTGGTCACTGGATGTGGACCCCGTGGATCTTTATTGA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5H365

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48