Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JJB06_RS02015 Genome accession   NZ_CP067342
Coordinates   442363..443040 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain ECJXMCRH7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 437363..448040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJB06_RS02000 (JJB06_01970) chiA 438730..441423 (+) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -
  JJB06_RS02005 (JJB06_01975) bfd 441592..441786 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JJB06_RS02010 (JJB06_01980) bfr 441858..442334 (+) 477 WP_000675504.1 bacterioferritin -
  JJB06_RS02015 (JJB06_01985) pilD 442363..443040 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JJB06_RS02020 (JJB06_01990) gspM 443040..443501 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JJB06_RS02025 (JJB06_01995) gspL 443498..444661 (-) 1164 Protein_404 type II secretion system protein GspL -
  JJB06_RS02030 (JJB06_02000) gspK 444676..445659 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  JJB06_RS02035 (JJB06_02005) gspJ 445652..446239 (-) 588 WP_000610366.1 type II secretion system minor pseudopilin GspJ -
  JJB06_RS02040 (JJB06_02010) gspI 446232..446609 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JJB06_RS02045 (JJB06_02015) gspH 446606..447115 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  JJB06_RS02050 (JJB06_02020) gspG 447123..447560 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=457936 JJB06_RS02015 WP_000178161.1 442363..443040(-) (pilD) [Escherichia coli strain ECJXMCRH7]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=457936 JJB06_RS02015 WP_000178161.1 442363..443040(-) (pilD) [Escherichia coli strain ECJXMCRH7]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378