Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HV340_RS20435 Genome accession   NZ_CP055883
Coordinates   4223611..4225044 (+) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter sp. RHBSTW-00975     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4218611..4230044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV340_RS20405 (HV340_20410) - 4219178..4220155 (+) 978 WP_020883528.1 calcium/sodium antiporter -
  HV340_RS20410 (HV340_20415) kdsD 4220169..4221155 (+) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  HV340_RS20415 (HV340_20420) kdsC 4221170..4221736 (+) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HV340_RS20420 (HV340_20425) lptC 4221733..4222308 (+) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  HV340_RS20425 (HV340_20430) lptA 4222277..4222831 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HV340_RS20430 (HV340_20435) lptB 4222838..4223563 (+) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  HV340_RS20435 (HV340_20440) rpoN/rpoN1 4223611..4225044 (+) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  HV340_RS20440 (HV340_20445) hpf 4225067..4225354 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  HV340_RS20445 (HV340_20450) ptsN 4225437..4225928 (+) 492 WP_181216199.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HV340_RS20450 (HV340_20455) rapZ 4225974..4226828 (+) 855 WP_014171820.1 RNase adapter RapZ -
  HV340_RS20455 (HV340_20460) npr 4226825..4227097 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  HV340_RS20460 (HV340_20465) mtgA 4227136..4227861 (-) 726 WP_014171821.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HV340_RS20465 (HV340_20470) elbB 4227858..4228511 (-) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=457869 HV340_RS20435 WP_014171817.1 4223611..4225044(+) (rpoN/rpoN1) [Enterobacter sp. RHBSTW-00975]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=457869 HV340_RS20435 WP_014171817.1 4223611..4225044(+) (rpoN/rpoN1) [Enterobacter sp. RHBSTW-00975]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCTTTCT
CCGATACCGACCGCGCGATTGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATTCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGCCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATTTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486