Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JJB24_RS19225 Genome accession   NZ_CP067299
Coordinates   3982194..3982871 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain ABW_A33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3977194..3987871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJB24_RS19190 (JJB24_19185) gspG 3977674..3978111 (+) 438 WP_032258448.1 type II secretion system major pseudopilin GspG -
  JJB24_RS19195 (JJB24_19190) gspH 3978119..3978628 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  JJB24_RS19200 (JJB24_19195) gspI 3978625..3979002 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JJB24_RS19205 (JJB24_19200) gspJ 3978995..3979582 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JJB24_RS19210 (JJB24_19205) gspK 3979575..3980558 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JJB24_RS19215 (JJB24_19210) gspL 3980573..3981736 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  JJB24_RS19220 (JJB24_19215) gspM 3981733..3982194 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  JJB24_RS19225 (JJB24_19220) pilD 3982194..3982871 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JJB24_RS19230 (JJB24_19225) bfr 3982900..3983376 (-) 477 WP_000675504.1 bacterioferritin -
  JJB24_RS19235 (JJB24_19230) bfd 3983448..3983642 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JJB24_RS19240 (JJB24_19235) chiA 3983811..3986504 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=457665 JJB24_RS19225 WP_000178161.1 3982194..3982871(+) (pilD) [Escherichia coli strain ABW_A33]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=457665 JJB24_RS19225 WP_000178161.1 3982194..3982871(+) (pilD) [Escherichia coli strain ABW_A33]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378