Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JJB13_RS16145 Genome accession   NZ_CP067265
Coordinates   3362597..3363274 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain ABW_S38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3357597..3368274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJB13_RS16130 (JJB13_16125) chiA 3358964..3361657 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  JJB13_RS16135 (JJB13_16130) bfd 3361826..3362020 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JJB13_RS16140 (JJB13_16135) bfr 3362092..3362568 (+) 477 WP_000675504.1 bacterioferritin -
  JJB13_RS16145 (JJB13_16140) pilD 3362597..3363274 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JJB13_RS16150 (JJB13_16145) gspM 3363274..3363735 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JJB13_RS16155 (JJB13_16150) gspL 3363732..3364895 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JJB13_RS16160 (JJB13_16155) gspK 3364910..3365893 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JJB13_RS16165 (JJB13_16160) gspJ 3365886..3366473 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JJB13_RS16170 (JJB13_16165) gspI 3366466..3366843 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JJB13_RS16175 (JJB13_16170) gspH 3366840..3367349 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  JJB13_RS16180 (JJB13_16175) gspG 3367357..3367794 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=457584 JJB13_RS16145 WP_000178161.1 3362597..3363274(-) (pilD) [Escherichia coli strain ABW_S38]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=457584 JJB13_RS16145 WP_000178161.1 3362597..3363274(-) (pilD) [Escherichia coli strain ABW_S38]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378