Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   HV559_RS05155 Genome accession   NZ_CP055306
Coordinates   1042364..1043677 (-) Length   437 a.a.
NCBI ID   WP_159629284.1    Uniprot ID   A0A7D5HTS3
Organism   Mannheimia pernigra strain BNO311     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037364..1048677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV559_RS05125 (HV559_05125) ruvB 1037993..1038997 (-) 1005 WP_159629278.1 Holliday junction branch migration DNA helicase RuvB -
  HV559_RS05130 (HV559_05130) ruvA 1039008..1039610 (-) 603 WP_176809724.1 Holliday junction branch migration protein RuvA -
  HV559_RS05135 (HV559_05135) - 1039719..1040345 (-) 627 WP_159629280.1 LysE family transporter -
  HV559_RS05140 (HV559_05140) - 1040339..1040821 (-) 483 WP_176808048.1 phosphatidylglycerophosphatase A -
  HV559_RS05145 (HV559_05145) thiL 1040827..1041807 (-) 981 WP_176809725.1 thiamine-phosphate kinase -
  HV559_RS05150 (HV559_05150) nusB 1041871..1042284 (-) 414 WP_159629283.1 transcription antitermination factor NusB -
  HV559_RS05155 (HV559_05155) comE 1042364..1043677 (-) 1314 WP_159629284.1 type IV pilus secretin PilQ Machinery gene
  HV559_RS05160 (HV559_05160) - 1043686..1044096 (-) 411 WP_176809726.1 hypothetical protein -
  HV559_RS05165 (HV559_05165) - 1044098..1044631 (-) 534 WP_176809727.1 hypothetical protein -
  HV559_RS05170 (HV559_05170) - 1044633..1045154 (-) 522 WP_176809728.1 hypothetical protein -
  HV559_RS05175 (HV559_05175) - 1045130..1045837 (-) 708 WP_176809729.1 competence protein ComA -
  HV559_RS05180 (HV559_05180) - 1045964..1048513 (+) 2550 WP_176808055.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48415.60 Da        Isoelectric Point: 8.9535

>NTDB_id=457527 HV559_RS05155 WP_159629284.1 1042364..1043677(-) (comE) [Mannheimia pernigra strain BNO311]
MFRIFLFLFISSSLAFANTISLAVKNAPTATILTYLSEEIGKNIVLDDNIQTKSTLRLENKSIDDIFKVISTMNKLSLHQ
EGDVIYISKKEEKLTDLKTIPIAHLQNNIENTPLVTTPKLITKTIKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSTKSLQHIEKLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSKGADHYKFGGRLESNGLSNVANNLNVS
FPVTNGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNKKSEATNVEFKEAVLG
LEVTPHLSTENQILLDLIVTQNSPNSQSGSSGLVTIDKQELNTQVFAKHGETIVLGGVFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYIIQSNEKLGGVKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=457527 HV559_RS05155 WP_159629284.1 1042364..1043677(-) (comE) [Mannheimia pernigra strain BNO311]
ATGTTTAGAATTTTTCTTTTTTTATTTATTAGCTCCTCATTAGCATTTGCTAACACAATTTCTCTTGCTGTGAAAAATGC
CCCCACTGCAACCATTTTGACTTATTTATCCGAAGAAATAGGAAAAAATATTGTTTTAGATGATAATATTCAAACAAAAT
CAACATTAAGATTAGAAAATAAATCCATTGACGATATATTCAAAGTAATAAGCACAATGAATAAATTATCGCTACATCAA
GAAGGTGATGTTATTTATATCAGTAAAAAAGAAGAAAAATTGACCGATTTAAAAACAATTCCAATCGCTCATTTGCAAAA
TAATATAGAAAATACACCGCTTGTTACCACACCCAAACTCATTACTAAAACAATAAAACTACATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACAAAAGGCAGCGGTACTTTTTTATCTGAAAATGGCTATATCCATTTTGATGAGCGAAGTAAT
AGTTTAATTATCAAAGACAGTACAAAATCACTTCAACACATAGAAAAGTTAGTTAAACAGCTTGACCAACCAACTGAGCA
AATTGCAATTGAAGCACGCATTGTCACAATCAGCAGTGAACATTTACAAGAATTGGGGGTGCGTTGGGGAATGTTCTCAA
AAGGGGCTGATCACTATAAATTTGGTGGGCGACTTGAAAGTAACGGATTAAGCAATGTTGCTAATAATCTCAATGTGAGT
TTTCCTGTTACGAATGGTGCTTCAGCAGTTTTACAAGTCGCTTCTATTAATAGCCGAGTACTGGATCTGGAATTGAGTGC
CTTAGAGCAAGAAAATAGTGTAGAAATTATCGCTAGCCCTCGCCTTTTGACTACGAATAAAAAACCAGCGAGTATCAAAC
AAGGTACAGAAATTCCTTATGTGATGTACAATAAAAAATCGGAAGCTACTAATGTGGAATTTAAAGAAGCCGTGCTGGGC
TTAGAAGTTACTCCTCATCTTTCGACTGAAAATCAAATATTACTCGATCTGATTGTTACTCAAAATTCTCCTAATTCGCA
ATCAGGCAGTAGTGGCTTAGTGACTATTGATAAACAAGAATTAAATACACAAGTTTTTGCGAAACACGGAGAAACAATCG
TTTTAGGCGGAGTTTTCCAACATTTAACGCAAAAAGGAGAAGATAAAGTACCTATTTTAGGTTCAATTCCGTTTATCAAA
CGCTTATTTAGCCAAACTCGAGATAAAATTAGCAAAAGAGAACTCGTGATTTTTGTTACCCCTTACATTATTCAATCAAA
TGAAAAATTAGGTGGTGTGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D5HTS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

61.839

99.542

0.616
  comE Haemophilus influenzae Rd KW20

49.433

100

0.499
  comE Haemophilus influenzae 86-028NP

49.206

100

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.858

97.025

0.387
  pilQ Vibrio cholerae strain A1552

39.858

97.025

0.387
  pilQ Vibrio campbellii strain DS40M4

38.695

98.169

0.38