Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HUK62_RS15300 Genome accession   NZ_CP055277
Coordinates   3161502..3162302 (-) Length   266 a.a.
NCBI ID   WP_004983514.1    Uniprot ID   A0A7H8SKN2
Organism   Acinetobacter sp. NEB 394     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3156502..3167302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK62_RS15280 (HUK62_15280) - 3157583..3158281 (-) 699 WP_005401704.1 tetratricopeptide repeat protein -
  HUK62_RS15285 (HUK62_15285) hisS 3158309..3159604 (-) 1296 WP_176538281.1 histidine--tRNA ligase -
  HUK62_RS15290 (HUK62_15290) ispG 3159601..3160716 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  HUK62_RS15295 (HUK62_15295) - 3160735..3161511 (-) 777 WP_176538282.1 helix-turn-helix domain-containing protein -
  HUK62_RS15300 (HUK62_15300) pilF 3161502..3162302 (-) 801 WP_004983514.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HUK62_RS15305 (HUK62_15305) rlmN 3162319..3163554 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  HUK62_RS15310 (HUK62_15310) ndk 3163677..3164108 (-) 432 WP_176538283.1 nucleoside-diphosphate kinase -
  HUK62_RS15315 (HUK62_15315) iscX 3164219..3164419 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  HUK62_RS15320 (HUK62_15320) - 3164510..3165061 (-) 552 WP_176538284.1 phosphatase PAP2 family protein -
  HUK62_RS15325 (HUK62_15325) - 3165254..3166534 (-) 1281 WP_176538285.1 glycosyltransferase family 1 protein -
  HUK62_RS15330 (HUK62_15330) - 3166675..3167091 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30016.01 Da        Isoelectric Point: 8.9759

>NTDB_id=457387 HUK62_RS15300 WP_004983514.1 3161502..3162302(-) (pilF) [Acinetobacter sp. NEB 394]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=457387 HUK62_RS15300 WP_004983514.1 3161502..3162302(-) (pilF) [Acinetobacter sp. NEB 394]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCTGAGCGTTATTTTAAGCATGCGATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAGAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCTCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAATACTATGAACAGTTTGTGCGAGCGGTGGGCGAGAAAAACTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACAATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8SKN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647