Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HUW50_RS09770 Genome accession   NZ_CP055263
Coordinates   2070664..2072037 (+) Length   457 a.a.
NCBI ID   WP_066330452.1    Uniprot ID   -
Organism   Metabacillus sp. KUDC1714     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2065664..2077037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW50_RS09750 (HUW50_09750) - 2065827..2066288 (+) 462 WP_066330456.1 CtsR family transcriptional regulator -
  HUW50_RS09755 (HUW50_09755) - 2066304..2066855 (+) 552 WP_066330455.1 UvrB/UvrC motif-containing protein -
  HUW50_RS09760 (HUW50_09760) - 2066858..2067955 (+) 1098 WP_066330454.1 protein arginine kinase -
  HUW50_RS09765 (HUW50_09765) clpC 2067952..2070399 (+) 2448 WP_066330453.1 ATP-dependent protease ATP-binding subunit ClpC -
  HUW50_RS09770 (HUW50_09770) radA 2070664..2072037 (+) 1374 WP_066330452.1 DNA repair protein RadA Machinery gene
  HUW50_RS09775 (HUW50_09775) disA 2072041..2073120 (+) 1080 WP_066330451.1 DNA integrity scanning diadenylate cyclase DisA -
  HUW50_RS09780 (HUW50_09780) - 2073478..2074575 (+) 1098 WP_066330450.1 PIN/TRAM domain-containing protein -
  HUW50_RS09785 (HUW50_09785) ispD 2074861..2075535 (+) 675 WP_066330449.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HUW50_RS09790 (HUW50_09790) ispF 2075728..2076207 (+) 480 WP_066330448.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49626.18 Da        Isoelectric Point: 7.8016

>NTDB_id=457306 HUW50_RS09770 WP_066330452.1 2070664..2072037(+) (radA) [Metabacillus sp. KUDC1714]
MAKTKVKFICQTCGYESPKWMGKCPGCGEWNTMTEEVLKPTSPRKAVFAHSTQTVQKPSPITNIETTQEPRIFTNLKEFN
RVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSSQLAENGNDVLYISGEESVKQTKLRADRLGVKANKLFVLSETDLSFISR
AIEETNPAFVVVDSIQTVFHSEISSAPGSVSQVRESTAELMRIAKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVQNPSEIFLEERSKGAAGSTVVASMEGTRPVLVEIQALISPTSFG
NPRRMATGIDHNRVPLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFKDAPPKPTDVIIGEVGLTG
EVRRVSRIEQRVMEAAKLGFKRAIIPEANIGGWNPPDDIEIIGVKNVAEALHKTLGG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=457306 HUW50_RS09770 WP_066330452.1 2070664..2072037(+) (radA) [Metabacillus sp. KUDC1714]
ATGGCTAAAACGAAGGTAAAGTTTATTTGTCAAACATGTGGATATGAATCACCTAAATGGATGGGGAAATGTCCGGGTTG
TGGTGAATGGAATACAATGACAGAGGAAGTTTTAAAGCCTACTTCACCTCGGAAGGCGGTTTTTGCTCATTCCACCCAAA
CGGTACAAAAGCCTTCTCCGATTACAAATATAGAAACAACCCAAGAACCAAGGATTTTTACAAATCTAAAAGAATTTAAC
CGTGTCTTAGGAAGCGGTATTGTTAAGGGCTCCCTTGTATTAATTGGTGGAGATCCTGGGATTGGCAAGTCTACACTTTT
ACTGCAAGTATCATCACAGTTAGCTGAAAATGGAAATGATGTATTGTATATTTCAGGAGAAGAATCAGTAAAACAAACAA
AACTAAGGGCTGACCGCTTAGGGGTTAAAGCAAATAAACTATTTGTTTTGTCTGAAACAGATTTAAGTTTTATTTCAAGG
GCGATTGAAGAAACAAACCCTGCATTTGTGGTTGTCGATTCAATCCAAACAGTATTCCATAGCGAAATAAGCTCTGCTCC
TGGAAGTGTGTCACAGGTAAGAGAATCTACTGCAGAACTTATGAGAATTGCGAAAACAAAAGGTATTGCCATCTTTATTG
TGGGTCATGTAACGAAAGAAGGGTCAATCGCTGGACCGAGATTATTGGAACATATGGTTGATACTGTTCTATATTTTGAA
GGAGAAAGACATCATACGTATCGTATATTACGTGCTGTAAAGAATCGGTTTGGTTCAACAAATGAAATGGGAATATTTGA
GATGAAGGAATCTGGTCTAGAAGAGGTGCAAAATCCCTCAGAAATATTTCTAGAAGAAAGATCAAAAGGCGCAGCTGGTT
CAACTGTTGTAGCTTCAATGGAGGGAACGAGACCTGTTCTTGTCGAGATTCAAGCCTTAATTTCACCAACTAGCTTCGGT
AACCCTAGAAGAATGGCAACAGGAATTGATCACAACCGTGTACCTTTATTAATGGCAGTTTTAGAAAAACGAGTTGGCTT
GCTTCTTCAAAATCAAGATGCGTATTTAAAAGTTGCTGGTGGAGTGAAGCTTGATGAACCTGCTATCGATTTAGCAGTTG
CTGTAAGCATTGCATCAAGTTTTAAGGATGCACCCCCAAAACCAACTGATGTCATTATTGGTGAAGTGGGGTTAACTGGT
GAAGTCCGTAGAGTTTCTAGGATTGAACAAAGAGTAATGGAGGCAGCAAAGCTTGGTTTTAAAAGGGCGATTATTCCAGA
GGCAAATATAGGTGGATGGAACCCTCCAGATGATATCGAGATTATTGGAGTGAAGAATGTAGCGGAGGCTCTCCATAAAA
CGTTAGGAGGATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

81.441

100

0.816
  radA Streptococcus mitis NCTC 12261

61.319

99.562

0.611
  radA Streptococcus mitis SK321

61.319

99.562

0.611
  radA Streptococcus pneumoniae Rx1

61.099

99.562

0.608
  radA Streptococcus pneumoniae D39

61.099

99.562

0.608
  radA Streptococcus pneumoniae R6

61.099

99.562

0.608
  radA Streptococcus pneumoniae TIGR4

61.099

99.562

0.608